Comparative Glycoproteome Analysis: Dynamics of Protein Glycosylation during Metamorphic Transition from Pelagic to Benthic Life Stages in Three Invertebrates

Type
Article

Authors
Chandramouli, Kondethimmanahalli
Zhang, Yu
Wong, Yue Him
Qian, Pei-Yuan

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)

KAUST Grant Number
SA-C0040
UK-00016

Online Publication Date
2011-12-15

Print Publication Date
2012-02-03

Date
2011-12-15

Abstract
The life cycle of most benthic marine invertebrates has two distinct stages: the pelagic larval stage and the sessile juvenile stage. The transition between the larval stage and the juvenile stage is often abrupt and may be triggered by post-translational modification of proteins. Glycosylation, a very important post-translational modification, influences the biological activity of proteins. We used two-dimensional gel electrophoresis (2-DE) followed by glycoprotein-specific fluorescence staining and mass spectrometry with the goal of identifying glycosylation pattern changes during larval settlement and metamorphosis in barnacles, bryozoans, and polychaetes. Our results revealed substantial changes in the protein glycosylation patterns from larval to juvenile stages. Before metamorphosis, the degree of protein glycosylation was high in the barnacle Balanus (=Amphibalanus) amphitrite and the spionid polychaete Pseudopolydora vexillosa, whereas it increased after metamorphosis in the bryozoan Bugula neritina. We identified 19 abundant and differentially glycosylated proteins in these three species. Among the proteins, cellular stress- and metabolism-related proteins exhibited distinct glycosylation in B. amphitrite and B. neritina, whereas fatty acid metabolism-related proteins were abundantly glycosylated in P. vexillosa. Furthermore, the protein and gene expression analysis of some selected glycoproteins revealed that the degree of protein glycosylation did not always complement with transcriptional and translational changes associated with the larval-juvenile transition. The current study provides preliminary information on protein glycosylation in marine invertebrates that will serve as a solid basis for future comprehensive analysis of glycobiology during larval settlement and metamorphosis. © 2011 American Chemical Society.

Citation
Chandramouli KH, Zhang Y, Wong YH, Qian P-Y (2012) Comparative Glycoproteome Analysis: Dynamics of Protein Glycosylation during Metamorphic Transition from Pelagic to Benthic Life Stages in Three Invertebrates. Journal of Proteome Research 11: 1330–1340. Available: http://dx.doi.org/10.1021/pr200982k.

Acknowledgements
The authors thank Dr. Flora S. Y. Mok for her help in collecting P. vexillosa larvae, Dr. Kiyotaka Matsumura for his critical review of the manuscript, and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from King Abdullah University of Science and Technology (SA-C0040/UK-00016) and a grant from the Research Grants Council of the Hong Kong Special Administrative Region (AoE/P-04/04-II) to P.-Y. Qian.

Publisher
American Chemical Society (ACS)

Journal
Journal of Proteome Research

DOI
10.1021/pr200982k

PubMed ID
22111546

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