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    Comparative Glycoproteome Analysis: Dynamics of Protein Glycosylation during Metamorphic Transition from Pelagic to Benthic Life Stages in Three Invertebrates

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    Type
    Article
    Authors
    Chandramouli, Kondethimmanahalli cc
    Zhang, Yu
    Wong, Yue Him
    Qian, Pei-Yuan cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Computational Bioscience Research Center (CBRC)
    KAUST Grant Number
    SA-C0040
    UK-00016
    Date
    2011-12-15
    Online Publication Date
    2011-12-15
    Print Publication Date
    2012-02-03
    Permanent link to this record
    http://hdl.handle.net/10754/600217
    
    Metadata
    Show full item record
    Abstract
    The life cycle of most benthic marine invertebrates has two distinct stages: the pelagic larval stage and the sessile juvenile stage. The transition between the larval stage and the juvenile stage is often abrupt and may be triggered by post-translational modification of proteins. Glycosylation, a very important post-translational modification, influences the biological activity of proteins. We used two-dimensional gel electrophoresis (2-DE) followed by glycoprotein-specific fluorescence staining and mass spectrometry with the goal of identifying glycosylation pattern changes during larval settlement and metamorphosis in barnacles, bryozoans, and polychaetes. Our results revealed substantial changes in the protein glycosylation patterns from larval to juvenile stages. Before metamorphosis, the degree of protein glycosylation was high in the barnacle Balanus (=Amphibalanus) amphitrite and the spionid polychaete Pseudopolydora vexillosa, whereas it increased after metamorphosis in the bryozoan Bugula neritina. We identified 19 abundant and differentially glycosylated proteins in these three species. Among the proteins, cellular stress- and metabolism-related proteins exhibited distinct glycosylation in B. amphitrite and B. neritina, whereas fatty acid metabolism-related proteins were abundantly glycosylated in P. vexillosa. Furthermore, the protein and gene expression analysis of some selected glycoproteins revealed that the degree of protein glycosylation did not always complement with transcriptional and translational changes associated with the larval-juvenile transition. The current study provides preliminary information on protein glycosylation in marine invertebrates that will serve as a solid basis for future comprehensive analysis of glycobiology during larval settlement and metamorphosis. © 2011 American Chemical Society.
    Citation
    Chandramouli KH, Zhang Y, Wong YH, Qian P-Y (2012) Comparative Glycoproteome Analysis: Dynamics of Protein Glycosylation during Metamorphic Transition from Pelagic to Benthic Life Stages in Three Invertebrates. Journal of Proteome Research 11: 1330–1340. Available: http://dx.doi.org/10.1021/pr200982k.
    Sponsors
    The authors thank Dr. Flora S. Y. Mok for her help in collecting P. vexillosa larvae, Dr. Kiyotaka Matsumura for his critical review of the manuscript, and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from King Abdullah University of Science and Technology (SA-C0040/UK-00016) and a grant from the Research Grants Council of the Hong Kong Special Administrative Region (AoE/P-04/04-II) to P.-Y. Qian.
    Publisher
    American Chemical Society (ACS)
    Journal
    Journal of Proteome Research
    DOI
    10.1021/pr200982k
    PubMed ID
    22111546
    ae974a485f413a2113503eed53cd6c53
    10.1021/pr200982k
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC); Publications Acknowledging KAUST Support

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