Changes in the proteome and phosphoproteome expression in the bryozoan Bugula neritina larvae in response to the antifouling agent butenolide
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AbstractLarval attachment and metamorphosis, commonly referred to as larval settlement, of marine sessile invertebrates can be triggered or blocked by chemical cues and affected by changes in overall protein expression pattern and phosphorylation dynamics. This study focuses on the effects of butenolide, an effective larval settlement inhibitor, on larval settlement at the proteome level in the bryozoan Bugula neritina. Liquid-phase IEF sample prefractionation combined with 2-DE and MALDI-TOF MS was used to identify the differentially expressed proteins. Substantial changes occurred both in protein abundance and in phosphorylation status during larval settlement and when settling larvae were challenged with butenolide. The proteins that responded to treatment were identified as structural proteins, molecular chaperones, mitochondrial peptidases and calcium-binding proteins. Compared with our earlier results, both genistein and butenolide inhibited larval settlement of B. neritina primarily by changes in protein abundance and the phosphorylation status of proteins but have different protein targets in the same species. Clearly, to design potent antifouling compounds and to understand the mode of action of compounds, more studies on the effects of different compounds on proteome and phosphoproteome of different larval species are required. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
CitationQian P-Y, Wong YH, Zhang Y (2010) Changes in the proteome and phosphoproteome expression in the bryozoan Bugula neritina larvae in response to the antifouling agent butenolide. PROTEOMICS 10: 3435–3446. Available: http://dx.doi.org/10.1002/pmic.201000199.
SponsorsThe authors thank Dr. Kondethimmanahalli Chandramouli for conducting most of the laboratory work and data analysis as well as helping with initial draft, Dr. Huoming Zhang and Dr. Shawn Arellano for proofreading the manuscript. This study was supported by award SA-C0040/UK-00016 from the King Abdullah University of Science and Technology, a grant from China Ocean Mineral Resource Research and Development Association (COMARRD06/07.Sc02), and grants (N-HKUST602/09, 662408 and AoE/P-04/04-II) from the Research Grants Council of the Hong Kong Special Administrative Region to P.-Y. Qian.
CollectionsPublications Acknowledging KAUST Support
- 2D gel-based proteome and phosphoproteome analysis during larval metamorphosis in two major marine biofouling invertebrates.
- Authors: Thiyagarajan V, Wong T, Qian PY
- Issue date: 2009 Jun
- The effect of butenolide on behavioral and morphological changes in two marine fouling species, the barnacle Balanus amphitrite and the bryozoan Bugula neritina.
- Authors: Zhang YF, Wang GC, Ying X, Sougrat R, Qian PY
- Issue date: 2011 May
- Quantitative proteomics identify molecular targets that are crucial in larval settlement and metamorphosis of Bugula neritina.
- Authors: Zhang H, Wong YH, Wang H, Chen Z, Arellano SM, Ravasi T, Qian PY
- Issue date: 2011 Jan 7
- Comparative proteome and phosphoproteome analyses during cyprid development of the barnacle Balanus (=Amphibalanus) amphitrite.
- Authors: Zhang Y, Xu Y, Arellano SM, Xiao K, Qian PY
- Issue date: 2010 Jun 4
- 2D gel-based multiplexed proteomic analysis during larval development and metamorphosis of the biofouling polychaete tubeworm Hydroides elegans.
- Authors: Zhang Y, Sun J, Xiao K, Arellano SM, Thiyagarajan V, Qian PY
- Issue date: 2010 Sep 3