Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally?
KAUST Grant NumberKUK-C1-013-04
Online Publication Date2010-02-05
Print Publication Date2010-06
Permanent link to this recordhttp://hdl.handle.net/10754/600194
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AbstractWe trace the history of attempts to determine whether the experimentally observed diminution of metabolic energy expenditure when muscles lengthen during active contraction is consistent with reversibility of biochemical reactions and, in particular, with the regeneration of ATP. We note that this scientific endeavor has something of a parallel flavor to it, with both early and more recent experiments exploiting both isolated muscle preparations and exercising human subjects. In tracing this history from the late 19th century to the present, it becomes clear that energy can be (at least transiently) stored in a muscle undergoing an eccentric contraction but that this is unlikely to be due to the regeneration of ATP. A recently developed, thermodynamically constrained model of the cross-bridge cycle provides additional insight into this conclusion. Copyright © 2010 the American Physiological Society.
CitationLoiselle DS, Tran K, Crampin EJ, Curtin NA (2010) Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally? Journal of Applied Physiology 108: 1465–1471. Available: http://dx.doi.org/10.1152/japplphysiol.01198.2009.
SponsorsThis work was supported by Grant UOA0607 from the Royal Society of New Zealand Marsden Fund. This publication is based on work (E.J. Crampin) supported in part by Award No KUK-C1-013-04, made by King Abdullah Univ. of Science and Technology (KAUST).
PublisherAmerican Physiological Society
JournalJournal of Applied Physiology
CollectionsPublications Acknowledging KAUST Support
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