Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids
KAUST Grant NumberSA-C0040
MetadataShow full item record
AbstractBiofouling is ubiquitous in marine environments, and the barnacle Balanus amphitrite is one of the most recalcitrant and aggressive biofoulers in tropical waters. Several natural antifoulants that were claimed to be non-toxic have been isolated in recent years, although the mechanism by which they inhibit fouling is yet to be investigated. Poly-ether B has shown promise in the non-toxic inhibition of larval barnacle attachment. Hence, in this study, multiplex two-dimensional electrophoresis (2-DE) was applied in conjunction with mass spectrometry to investigate the effects of poly-ether B on barnacle larvae at the molecular level. The cyprid proteome response to poly-ether B treatment was analyzed at the total proteome and phosphoproteome levels, with 65 protein and 19 phosphoprotein spots found to be up- or down-regulated. The proteins were found to be related to energy-metabolism, oxidative stress, and molecular chaperones, thus indicating that poly-ether B may interfere with the redox-regulatory mechanisms governing the settlement of barnacle larvae. The results of this study demonstrate the usefulness of the proteomic technique in revealing the working mechanisms of antifouling compounds. © 2012 Copyright Taylor and Francis Group, LLC.
CitationDash S, Chandramouli KH, Zhang Y, Qian P-Y (2012) Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids . Biofouling 28: 405–415. Available: http://dx.doi.org/10.1080/08927014.2012.679731.
SponsorsThe authors thank Ms Cherry Kwan for proof reading this manuscript, Dr Wu Huixiang for her kind help with the barnacle culture, Dr Wang Yong for help in the preparation of the figures and Dr Ying Xu for her helpful suggestions. The study was supported by an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology (KAUST), a grant from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), and two grants (N_HKUST602/09 and AoE/P-393 04/04-2-II) from the Hong Kong Research Grants Council (RGC) awarded to PY Qian.
PublisherInforma UK Limited
CollectionsPublications Acknowledging KAUST Support
- Proteomic analysis of larvae during development, attachment, and metamorphosis in the fouling barnacle, Balanus amphitrite.
- Authors: Thiyagarajan V, Qian PY
- Issue date: 2008 Aug
- Comparative proteome and phosphoproteome analyses during cyprid development of the barnacle Balanus (=Amphibalanus) amphitrite.
- Authors: Zhang Y, Xu Y, Arellano SM, Xiao K, Qian PY
- Issue date: 2010 Jun 4
- 2D gel-based proteome and phosphoproteome analysis during larval metamorphosis in two major marine biofouling invertebrates.
- Authors: Thiyagarajan V, Wong T, Qian PY
- Issue date: 2009 Jun
- iTRAQ-based proteomic profiling of the barnacle Balanus amphitrite in response to the antifouling compound meleagrin.
- Authors: Han Z, Sun J, Zhang Y, He F, Xu Y, Matsumura K, He LS, Qiu JW, Qi SH, Qian PY
- Issue date: 2013 May 3
- Modulation of barnacle (Balanus amphitrite Darwin) cyprid settlement behavior by sulfobetaine and carboxybetaine methacrylate polymer coatings.
- Authors: Aldred N, Li G, Gao Y, Clare AS, Jiang S
- Issue date: 2010 Aug