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    Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite

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    Type
    Article
    Authors
    Zhang, Yu
    Xu, Ying
    Arellano, Shawn M.
    Xiao, Kang
    Qian, Pei-Yuan cc
    KAUST Grant Number
    SA-C0040
    UK-C0016
    Date
    2010-06-04
    Permanent link to this record
    http://hdl.handle.net/10754/600111
    
    Metadata
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    Abstract
    The barnacle Balanus amphitrite (=Amphibalanus amphitrite) is a major marine biofouling invertebrate worldwide. It has a complex life cycle during which the larva (called a nauplius) molts six times before transforming into the cyprid stage. The cyprid stage in B. amphitrite is the critical stage for the larval decision to attach and metamorphose. In this study, proteome and phosphoproteome alterations during cyprid development/aging and upon treatment with the antifouling agent butenolide were examined with a two-dimensional electrophoresis (2-DE) multiplexed fluorescent staining approach. Optimized protein separation strategies, including solution-phase isoelectric fractionation and narrow-pH-range 2-DE, were used in a proteomic analysis. Our results show that the differential regulation of the target proteins is highly dynamic on the levels of both protein expression and posttranslational modification. Two groups of proteins, stress-associated and energy metabolism-related proteins, are differentially expressed during cyprid development. Comparison of the control and treatment groups suggests that butenolide exerts its effects by sustaining the expression levels of these proteins. Altogether, our data suggest that proteins involved in stress regulation and energy metabolism play crucial roles in regulating larval attachment and metamorphosis of B. amphitrite. © 2010 American Chemical Society.
    Citation
    Zhang Y, Xu Y, Arellano SM, Xiao K, Qian P-Y (2010) Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite . Journal of Proteome Research 9: 3146–3157. Available: http://dx.doi.org/10.1021/pr1000384.
    Sponsors
    We thank Dr. Kondethimmanahalli Chandramouli for conducting sample fractionation and data analysis, Prof. Donald Choy Chang for providing the Sensor-C3 stably expressed HeLa cells (S3), Dr. Huoming Zhang for proof-reading the manuscript, and the rest of the laboratory team for their constructive comments on this work. This work was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.Sc02), an award (SA-C0040/UK-C0016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/P-04/04-II) of the government of the Hong Kong Special Administrative Region to PY Qian.
    Publisher
    American Chemical Society (ACS)
    Journal
    Journal of Proteome Research
    DOI
    10.1021/pr1000384
    PubMed ID
    20397722
    ae974a485f413a2113503eed53cd6c53
    10.1021/pr1000384
    Scopus Count
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