Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite
MetadataShow full item record
AbstractThe barnacle Balanus amphitrite (=Amphibalanus amphitrite) is a major marine biofouling invertebrate worldwide. It has a complex life cycle during which the larva (called a nauplius) molts six times before transforming into the cyprid stage. The cyprid stage in B. amphitrite is the critical stage for the larval decision to attach and metamorphose. In this study, proteome and phosphoproteome alterations during cyprid development/aging and upon treatment with the antifouling agent butenolide were examined with a two-dimensional electrophoresis (2-DE) multiplexed fluorescent staining approach. Optimized protein separation strategies, including solution-phase isoelectric fractionation and narrow-pH-range 2-DE, were used in a proteomic analysis. Our results show that the differential regulation of the target proteins is highly dynamic on the levels of both protein expression and posttranslational modification. Two groups of proteins, stress-associated and energy metabolism-related proteins, are differentially expressed during cyprid development. Comparison of the control and treatment groups suggests that butenolide exerts its effects by sustaining the expression levels of these proteins. Altogether, our data suggest that proteins involved in stress regulation and energy metabolism play crucial roles in regulating larval attachment and metamorphosis of B. amphitrite. © 2010 American Chemical Society.
CitationZhang Y, Xu Y, Arellano SM, Xiao K, Qian P-Y (2010) Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite . Journal of Proteome Research 9: 3146–3157. Available: http://dx.doi.org/10.1021/pr1000384.
SponsorsWe thank Dr. Kondethimmanahalli Chandramouli for conducting sample fractionation and data analysis, Prof. Donald Choy Chang for providing the Sensor-C3 stably expressed HeLa cells (S3), Dr. Huoming Zhang for proof-reading the manuscript, and the rest of the laboratory team for their constructive comments on this work. This work was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.Sc02), an award (SA-C0040/UK-C0016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/P-04/04-II) of the government of the Hong Kong Special Administrative Region to PY Qian.
PublisherAmerican Chemical Society (ACS)
JournalJournal of Proteome Research
CollectionsPublications Acknowledging KAUST Support
- Proteomic analysis of larvae during development, attachment, and metamorphosis in the fouling barnacle, Balanus amphitrite.
- Authors: Thiyagarajan V, Qian PY
- Issue date: 2008 Aug
- 2D gel-based proteome and phosphoproteome analysis during larval metamorphosis in two major marine biofouling invertebrates.
- Authors: Thiyagarajan V, Wong T, Qian PY
- Issue date: 2009 Jun
- iTRAQ-based proteomic profiling of the barnacle Balanus amphitrite in response to the antifouling compound meleagrin.
- Authors: Han Z, Sun J, Zhang Y, He F, Xu Y, Matsumura K, He LS, Qiu JW, Qi SH, Qian PY
- Issue date: 2013 May 3
- Response of larval barnacle proteome to CO(2)-driven seawater acidification.
- Authors: Wong KK, Lane AC, Leung PT, Thiyagarajan V
- Issue date: 2011 Sep
- Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids.
- Authors: Dash S, Chandramouli KH, Zhang Y, Qian PY
- Issue date: 2012