Toward a better understanding of the interaction between TGF-β family members and their ALK receptors
KAUST Grant NumberKUK-I1-012-43
Permanent link to this recordhttp://hdl.handle.net/10754/600043
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AbstractTransforming growth factor-beta (TGF-β) proteins are a family of structurally related extracellular proteins that trigger their signaling functions through interaction with the extracellular domains of their cognate serine/threonine kinase receptors. The specificity of TGF-β/receptor binding is complex and gives rise to multiple functional roles. Additionally, it is not completely understood at the atomic level. Here, we use the most reliable computational methods currently available to study systems involving activin-like kinase (ALK) receptors ALK4 and ALK7 and their multiple TGF-β ligands. We built models for all these proteins and their complexes for which experimental structures are not available. By analyzing the surfaces of interaction in six different TGF-β/ALK complexes we could infer which are the structural distinctive features of the ligand-receptor binding mode. Furthermore, this study allowed us to rationalize why binding of the growth factors GDF3 and Nodal to the ALK4 receptor requires the Cripto co-factor, whilst binding to the ALK7 receptor does not. © Springer-Verlag 2012.
CitationRomano V, Raimondo D, Calvanese L, D’Auria G, Tramontano A, et al. (2012) Toward a better understanding of the interaction between TGF-β family members and their ALK receptors. J Mol Model 18: 3617–3625. Available: http://dx.doi.org/10.1007/s00894-012-1370-y.
SponsorsKing Abdullah University of Science and Technology (KAUST; Award No. KUK-I1-012-43); Fondazione Roma and the Italian Ministry of Health (contract no. onc_ord 25/07, FIRB ITAL-BIONET and PROTEOMICA).Ministero dell'Universita e della Ricerca Scientifica (MIUR), project PRIN no prot. 2008F5A3AF_001.
JournalJournal of Molecular Modeling
CollectionsPublications Acknowledging KAUST Support
- Conformational features and binding affinities to Cripto, ALK7 and ALK4 of Nodal synthetic fragments.
- Authors: Calvanese L, Sandomenico A, Caporale A, Focà A, Focà G, D'Auria G, Falcigno L, Ruvo M
- Issue date: 2015 Apr
- Identification of a functional binding site for activin on the type I receptor ALK4.
- Authors: Harrison CA, Gray PC, Koerber SC, Fischer W, Vale W
- Issue date: 2003 Jun 6
- BMP-2 and BMP-9 binding specificities with ALK-3 in aqueous solution with dynamics.
- Authors: Coskuner O, Uversky VN
- Issue date: 2017 Oct
- Cripto forms a complex with activin and type II activin receptors and can block activin signaling.
- Authors: Gray PC, Harrison CA, Vale W
- Issue date: 2003 Apr 29
- Structural investigations on the Nodal-Cripto binding: a theoretical and experimental approach.
- Authors: Calvanese L, Marasco D, Doti N, Saporito A, D'Auria G, Paolillo L, Ruvo M, Falcigno L
- Issue date: 2010 Nov