Show simple item record

dc.contributor.authorQuitterer, Felix
dc.contributor.authorBeck, Philipp
dc.contributor.authorBacher, Adelbert
dc.contributor.authorGroll, Michael
dc.date.accessioned2016-02-28T06:32:20Z
dc.date.available2016-02-28T06:32:20Z
dc.date.issued2014-06-10
dc.identifier.citationQuitterer F, Beck P, Bacher A, Groll M (2014) The Formation of Pyrroline and Tetrahydropyridine Rings in Amino Acids Catalyzed by Pyrrolysine Synthase (PylD). Angew Chem Int Ed 53: 8150–8153. Available: http://dx.doi.org/10.1002/anie.201402595.
dc.identifier.issn1433-7851
dc.identifier.pmid24916332
dc.identifier.doi10.1002/anie.201402595
dc.identifier.urihttp://hdl.handle.net/10754/599914
dc.description.abstractThe dehydrogenase PylD catalyzes the ultimate step of the pyrrolysine pathway by converting the isopeptide L-lysine-Nε-3R-methyl-D-ornithine to the 22nd proteinogenic amino acid. In this study, we demonstrate how PylD can be harnessed to oxidize various isopeptides to novel amino acids by combining chemical synthesis with enzyme kinetics and X-ray crystallography. The data enable a detailed description of the PylD reaction trajectory for the biosynthesis of pyrroline and tetrahydropyridine rings as constituents of pyrrolysine analogues. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.description.sponsorshipThis work was supported by the Hans-Fischer-Gesellschaft, by Award No. FIC/2010/07 from KAUST, and by the Deutsche Forschungsgemeinschaft (DFG, grant GR1861/7-1).
dc.publisherWiley
dc.subjectamino acids
dc.subjectdehydrogenases
dc.subjectenzyme catalysis
dc.subjectprotein crystallography
dc.subjectpyrrolysine
dc.titleThe Formation of Pyrroline and Tetrahydropyridine Rings in Amino Acids Catalyzed by Pyrrolysine Synthase (PylD)
dc.typeArticle
dc.identifier.journalAngewandte Chemie International Edition
dc.contributor.institutionTechnische Universitat Munchen, Munich, Germany
kaust.grant.numberFIC/2010/07
dc.date.published-online2014-06-10
dc.date.published-print2014-07-28


This item appears in the following Collection(s)

Show simple item record