siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
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AbstractRNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
CitationZhang G, He L-S, Wong YH, Yu L, Qian P-Y (2015) siRNA transfection in larvae of the barnacle Amphibalanus amphitrite. Journal of Experimental Biology 218: 2505–2509. Available: http://dx.doi.org/10.1242/jeb.120113.
SponsorsThis work was supported by grants from China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611 and GRF662413) to P.-Y.Q. as well as the National Natural Science Foundation of China (31460092) to L.-S.H.
PublisherThe Company of Biologists
JournalJournal of Experimental Biology
CollectionsPublications Acknowledging KAUST Support
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