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    siRNA transfection in larvae of the barnacle Amphibalanus amphitrite

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    Type
    Article
    Authors
    Zhang, G.
    He, L.-S.
    Wong, Y. H.
    Yu, L.
    Qian, P.-Y.
    KAUST Grant Number
    SA-C0040
    UK-C0016
    Date
    2015-06-25
    Online Publication Date
    2015-06-25
    Print Publication Date
    2015-08-01
    Permanent link to this record
    http://hdl.handle.net/10754/599643
    
    Metadata
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    Abstract
    RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
    Citation
    Zhang G, He L-S, Wong YH, Yu L, Qian P-Y (2015) siRNA transfection in larvae of the barnacle Amphibalanus amphitrite. Journal of Experimental Biology 218: 2505–2509. Available: http://dx.doi.org/10.1242/jeb.120113.
    Sponsors
    This work was supported by grants from China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611 and GRF662413) to P.-Y.Q. as well as the National Natural Science Foundation of China (31460092) to L.-S.H.
    Publisher
    The Company of Biologists
    Journal
    Journal of Experimental Biology
    DOI
    10.1242/jeb.120113
    PubMed ID
    26113139
    ae974a485f413a2113503eed53cd6c53
    10.1242/jeb.120113
    Scopus Count
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