Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface
Type
ArticleAuthors
Capriotti, Anna LauraCaracciolo, Giulio
Cavaliere, Chiara
Crescenzi, Carlo
Pozzi, Daniela
Laganà, Aldo
KAUST Grant Number
KUK-F1-036-32Date
2011-07-02Online Publication Date
2011-07-02Print Publication Date
2011-09Permanent link to this record
http://hdl.handle.net/10754/599364
Metadata
Show full item recordAbstract
The knowledge about the interaction between plasma proteins and nanocarriers employed for in vivo delivery is fundamental to understand their biodistribution. Protein adsorption onto nanoparticle surface (protein corona) is strongly affected by vector surface characteristics. In general, the primary interaction is thought to be electrostatic, thus surface charge of carrier is supposed to play a central role in protein adsorption. Because protein corona composition can be critical in modifying the interactive surface that is recognized by cells, characterizing its formation onto lipid particles may serve as a fundamental predictive model for the in vivo efficiency of a lipidic vector. In the present work, protein coronas adsorbed onto three differently charged cationic liposome formulations were compared by a shotgun proteomic approach based on nano-liquid chromatography-high-resolution mass spectrometry. About 130 proteins were identified in each corona, with only small differences between the different cationic liposome formulations. However, this study could be useful for the future controlled design of colloidal drug carriers and possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins into body fluids. © 2011 Springer-Verlag.Citation
Capriotti AL, Caracciolo G, Cavaliere C, Crescenzi C, Pozzi D, et al. (2011) Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface. Analytical and Bioanalytical Chemistry 401: 1195–1202. Available: http://dx.doi.org/10.1007/s00216-011-5188-8.Sponsors
This publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).Publisher
Springer NaturePubMed ID
21725631ae974a485f413a2113503eed53cd6c53
10.1007/s00216-011-5188-8
Scopus Count
Collections
Publications Acknowledging KAUST SupportRelated articles
- DNA affects the composition of lipoplex protein corona: a proteomics approach.
- Authors: Capriotti AL, Caracciolo G, Caruso G, Foglia P, Pozzi D, Samperi R, Laganà A
- Issue date: 2011 Aug
- Analysis of plasma protein adsorption onto DC-Chol-DOPE cationic liposomes by HPLC-CHIP coupled to a Q-TOF mass spectrometer.
- Authors: Capriotti AL, Caracciolo G, Caruso G, Cavaliere C, Pozzi D, Samperi R, Laganà A
- Issue date: 2010 Dec
- Do plasma proteins distinguish between liposomes of varying charge density?
- Authors: Capriotti AL, Caracciolo G, Cavaliere C, Foglia P, Pozzi D, Samperi R, Laganà A
- Issue date: 2012 Mar 16
- Differential analysis of "protein corona" profile adsorbed onto different nonviral gene delivery systems.
- Authors: Capriotti AL, Caracciolo G, Caruso G, Foglia P, Pozzi D, Samperi R, Laganà A
- Issue date: 2011 Dec 15
- In Vivo Biomolecule Corona around Blood-Circulating, Clinically Used and Antibody-Targeted Lipid Bilayer Nanoscale Vesicles.
- Authors: Hadjidemetriou M, Al-Ahmady Z, Mazza M, Collins RF, Dawson K, Kostarelos K
- Issue date: 2015 Aug 25