Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment
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ArticleDate
2012-04-09Online Publication Date
2012-04-09Print Publication Date
2012-04-24Permanent link to this record
http://hdl.handle.net/10754/599360
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We report that short, synthetic, double- as well as single-stranded DNA can be aligned in stretched humid poly(vinyl alcohol) (PVA) matrix, and the secondary structure (nucleobase orientation) can be characterized with linear dichroism (LD) spectroscopy. Oligonucleotides of lengths varying between 10 (3.4 nm) and 60 bases (20.4 nm) were investigated with respect to structural properties in the gel-like polymer environment. The DNA conformation as a function of relative humidity reveals a strong dependence of helical structure of DNA on PVA hydration level, results of relevance for nanotechnical studies of DNA-based supramolecular systems. Also, the PVA gel could provide possibilities to test models for nucleic acid interactions and distribution in cell contexts, including structural stability of genetic material in the cell and PVA-packaging for gene delivery. A method by which duplex oligonucleotides, with sequences designed to provide specific binding sites, become amenable to polarized-light spectroscopy opens up new possibilities for studying structure in DNA complexes with small adduct molecules as well as proteins. © 2012 American Chemical Society.Citation
Hanczyc P, Åkerman B, Nordén B (2012) Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment. Langmuir 28: 6662–6669. Available: http://dx.doi.org/10.1021/la300960q.Sponsors
We thank Bobo Feng for discussions and useful comments on the manuscript. The research was supported by grants from the King Abdullah University of Science and Technology (KAUST) and the European Research Council (ERC) to B.N.Publisher
American Chemical Society (ACS)Journal
LangmuirPubMed ID
22452613ae974a485f413a2113503eed53cd6c53
10.1021/la300960q
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