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    Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae

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    Type
    Article
    Authors
    Barbrook, Adrian C.
    Dorrell, Richard G.
    Burrows, Jennifer
    Plenderleith, Lindsey J.
    Nisbet, R. Ellen R.
    Howe, Christopher J.
    Date
    2012-05-05
    Online Publication Date
    2012-05-05
    Print Publication Date
    2012-07
    Permanent link to this record
    http://hdl.handle.net/10754/599337
    
    Metadata
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    Abstract
    Although transcription and transcript processing in the chloroplasts of plants have been extensively characterised, the RNA metabolism of other chloroplast lineages across the eukaryotes remains poorly understood. In this paper, we use RT-PCR to study transcription and transcript processing in the chloroplasts of Amphidinium carterae, a model peridinin-containing dinoflagellate. These organisms have a highly unusual chloroplast genome, with genes located on multiple small 'minicircle' elements, and a number of idiosyncratic features of RNA metabolism including transcription via a rolling circle mechanism, and 3′ terminal polyuridylylation of transcripts. We demonstrate that transcription occurs in A. carterae via a rolling circle mechanism, as previously shown in the dinoflagellate Heterocapsa, and present evidence for the production of both polycistronic and monocistronic transcripts from A. carterae minicircles, including several regions containing ORFs previously not known to be expressed. We demonstrate the presence of both polyuridylylated and non-polyuridylylated transcripts in A. carterae, and show that polycistronic transcripts can be terminally polyuridylylated. We present a model for RNA metabolism in dinoflagellate chloroplasts where long polycistronic precursors are processed to form mature transcripts. Terminal polyuridylylation may mark transcripts with the correct 3′ end. © 2012 Springer Science+Business Media B.V.
    Citation
    Barbrook AC, Dorrell RG, Burrows J, Plenderleith LJ, Nisbet RER, et al. (2012) Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae. Plant Molecular Biology 79: 347–357. Available: http://dx.doi.org/10.1007/s11103-012-9916-z.
    Sponsors
    We thank the Leverhulme Trust (F/09 641/F), the Isaac Newton Trust (University of Cambridge), and the King Abdullah University of Science and Technology for financial support. R.G.D. was supported by a BBSRC PhD studentship, and C.J.H. by a University of South Australia Distinguished Researcher Award. We thank the anonymous referees for their helpful comments on the drafting of the manuscript.
    Publisher
    Springer Nature
    Journal
    Plant Molecular Biology
    DOI
    10.1007/s11103-012-9916-z
    PubMed ID
    22562591
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11103-012-9916-z
    Scopus Count
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