Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation.
MetadataShow full item record
AbstractTranscriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.
CitationBallarino M, Cazzella V, D’Andrea D, Grassi L, Bisceglie L, et al. (2014) Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation. . Mol Cell Biol 35: MCB.01394–14. Available: http://dx.doi.org/10.1128/MCB.01394-14.
SponsorsThis work was partially supported by grants from ERC (ERC-2013-AdG340172-MUNCODD), Telethon (GGP11149), the Epigen-Epigenomics Flagship Project, Parent Project Italia, and PRIN to I.B. and KAUST and PRIN to A.T.
PublisherAmerican Society for Microbiology
JournalMolecular and Cellular Biology
PubMed Central IDPMC4301723
CollectionsPublications Acknowledging KAUST Support
- The developmental transcriptome sequencing of bovine skeletal muscle reveals a long noncoding RNA, lncMD, promotes muscle differentiation by sponging miR-125b.
- Authors: Sun X, Li M, Sun Y, Cai H, Lan X, Huang Y, Bai Y, Qi X, Chen H
- Issue date: 2016 Nov
- Sirt1 AS lncRNA interacts with its mRNA to inhibit muscle formation by attenuating function of miR-34a.
- Authors: Wang GQ, Wang Y, Xiong Y, Chen XC, Ma ML, Cai R, Gao Y, Sun YM, Yang GS, Pang WJ
- Issue date: 2016 Feb 23
- Opposing roles of miR-21 and miR-29 in the progression of fibrosis in Duchenne muscular dystrophy.
- Authors: Zanotti S, Gibertini S, Curcio M, Savadori P, Pasanisi B, Morandi L, Cornelio F, Mantegazza R, Mora M
- Issue date: 2015 Jul
- Loss of miR-29 in myoblasts contributes to dystrophic muscle pathogenesis.
- Authors: Wang L, Zhou L, Jiang P, Lu L, Chen X, Lan H, Guttridge DC, Sun H, Wang H
- Issue date: 2012 Jun
- A lncRNA promotes myoblast proliferation by up-regulating GH1.
- Authors: Yue Y, Jin C, Chen M, Zhang L, Liu X, Ma W, Guo H
- Issue date: 2017 Sep