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dc.contributor.authorLundberg, Erik P.
dc.contributor.authorPlesa, Calin
dc.contributor.authorWilhelmsson, L. Marcus
dc.contributor.authorLincoln, Per
dc.contributor.authorBrown, Tom
dc.contributor.authorNordén, Bengt
dc.date.accessioned2016-02-25T13:44:02Z
dc.date.available2016-02-25T13:44:02Z
dc.date.issued2011-08-15
dc.identifier.citationLundberg EP, Plesa C, Wilhelmsson LM, Lincoln P, Brown T, et al. (2011) Nanofabrication Yields. Hybridization and Click-Fixation of Polycyclic DNA Nanoassemblies. ACS Nano 5: 7565–7575. Available: http://dx.doi.org/10.1021/nn202568q.
dc.identifier.issn1936-0851
dc.identifier.issn1936-086X
dc.identifier.pmid21827213
dc.identifier.doi10.1021/nn202568q
dc.identifier.urihttp://hdl.handle.net/10754/598935
dc.description.abstractWe demonstrate the stepwise assembly of a fully addressable polycyclic DNA hexagon nanonetwork for the preparation of a four-ring system, one of the biggest networks yet constructed from tripodal building blocks. We find that the yield exhibits a distinct upper level <100%, a fundamental problem of thermodynamic DNA assembly that appears to have been overlooked in the DNA nanotechnology literature. A simplistic model based on a single step-yield parameter y can quantitatively describe the total yield of DNA assemblies in one-pot reactions as Y = yduplex n, with n the number of hybridization steps. Experimental errors introducing deviations from perfect stoichiometry and the thermodynamics of hybridization equilibria contribute to decreasing the value of yduplex (on average y = 0.96 for our 10 base pair hybridization). For the four-ring system (n = 31), the total yield is thus less than 30%, which is clearly unsatisfactory if bigger nanoconstructs of this class are to be designed. Therefore, we introduced site-specific click chemistry for making and purifying robust building blocks for future modular constructs of larger assemblies. Although the present yield of this robust module was only about 10%, it demonstrates a first step toward a general fabrication approach. Interestingly, we find that the click yields follow quantitatively a binomial distribution, the predictability of which indicates the usefulness of preparing pools of pure and robust building blocks in this way. The binomial behavior indicates that there is no interference between the six simultaneous click reactions but that step-yield limiting factors such as topological constraints and Cu(I) catalyst concentration are local and independent. © 2011 American Chemical Society.
dc.description.sponsorshipThis research is funded by the European Research Council (ERC grant to B.N.) and King Abdullah University of Science and Technology (KAUST awardee B.N.).
dc.publisherAmerican Chemical Society (ACS)
dc.subjectclick chemistry
dc.subjectDNA nanostructure
dc.subjectfixation technology
dc.subjectsupramolecular assembly
dc.subjectyield analysis
dc.titleNanofabrication Yields. Hybridization and Click-Fixation of Polycyclic DNA Nanoassemblies
dc.typeArticle
dc.identifier.journalACS Nano
dc.contributor.institutionChalmers University of Technology, Göteborg, Sweden
dc.contributor.institutionUniversity of Southampton, Southampton, United Kingdom
dc.date.published-online2011-08-15
dc.date.published-print2011-09-27


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