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dc.contributor.authorXue, Zheng
dc.contributor.authorLu, Huijie
dc.contributor.authorLiu, Wen-Tso
dc.date.accessioned2016-02-25T13:41:22Z
dc.date.available2016-02-25T13:41:22Z
dc.date.issued2014-07-15
dc.identifier.citationXue Z, Lu H, Liu W-T (2014) Membrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community. Biofouling 30: 813–821. Available: http://dx.doi.org/10.1080/08927014.2014.935937.
dc.identifier.issn0892-7014
dc.identifier.issn1029-2454
dc.identifier.pmid25115516
dc.identifier.doi10.1080/08927014.2014.935937
dc.identifier.urihttp://hdl.handle.net/10754/598795
dc.description.abstractEnsuring the quality and reproducibility of results from biofilm structure and microbial community analysis is essential to membrane biofouling studies. This study evaluated the impacts of three sample preparation factors (ie number of buffer rinses, storage time at 4°C, and DNA extraction method) on the downstream analysis of nitrifying biofilms grown on ultrafiltration membranes. Both rinse and storage affected biofilm structure, as suggested by their strong correlation with total biovolume, biofilm thickness, roughness and the spatial distribution of EPS. Significant variations in DNA yields and microbial community diversity were also observed among samples treated by different rinses, storage and DNA extraction methods. For the tested biofilms, two rinses, no storage and DNA extraction with both mechanical and chemical cell lysis from attached biofilm were the optimal sample preparation procedures for obtaining accurate information about biofilm structure, EPS distribution and the microbial community. © 2014 © 2014 Taylor & Francis.
dc.description.sponsorshipThis study was supported by King Abdullah University of Science and Technology (KAUST, Thuwal, Saudi Arabia). The authors would like to thank Chris Wright at the University of Illinois at Urbana-Champaign Roy J. Carver Biotechnology Center for performing the Illumina MiSeq sequencing. The flow cells were kindly provided by Dr Peiying Hong and Moustapha Harb at the KAUST Water Desalination Reuse Center.
dc.publisherInforma UK Limited
dc.subjectbiofilm sample preparation
dc.subjectbiofouling
dc.subjectconfocal microscopy
dc.subjectextracellular polymeric substances
dc.subjectMiseq sequencing
dc.titleMembrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community
dc.typeArticle
dc.identifier.journalBiofouling
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, United States
dc.date.published-online2014-07-15
dc.date.published-print2014-08-09


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