Major similarities in the bacterial communities associated with lesioned and healthy Fungiidae corals
KAUST Grant NumberUSA 00002
Online Publication Date2013-03-21
Print Publication Date2013-07
Permanent link to this recordhttp://hdl.handle.net/10754/598752
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AbstractCultivation-based studies have demonstrated that yellow-band disease (YBD), a lesion-producing ailment affecting diverse species of coral, is caused by a consortium of Vibrio spp. This study takes the first cultivation-independent approach to examine the whole bacterial community associated with YBD-like lesioned corals. Two species of Fungiidae corals, Ctenactis crassa and Herpolitha limax, displaying YBD-like lesions were examined across diverse reefs throughout the Red Sea. Using a pyrosequencing approach targeting the V1-V3 regions of the SSU rRNA gene, no major differences in bacterial community composition or diversity were identified between healthy and lesioned corals of either species. Indicator species analysis did not find Vibrio significantly associated with the lesioned corals. However, operational taxonomic units belonging to the Ruegeria genus of Alphaproteobacteria and NS9 marine group of Flavobacteria were significantly associated with the lesioned corals. The most striking trend of this dataset was that reef location was found to be the most significant influence on the coral-bacterial community. It is possible that more pronounced lesion-specific bacterial signatures might have been concealed by the strong influence of environmental conditions on coral-bacteria. Overall, this study demonstrates inconsistencies between cultivation-independent and cultivation-based studies regarding the role of specific bacteria in coral diseases. © 2013 John Wiley & Sons Ltd and Society for Applied Microbiology.
CitationApprill A, Hughen K, Mincer T (2013) Major similarities in the bacterial communities associated with lesioned and healthy Fungiidae corals. Environmental Microbiology 15: 2063–2072. Available: http://dx.doi.org/10.1111/1462-2920.12107.
SponsorsWe are grateful to J. Cervino for lesion identification, K. Furby, J. Ossolinski, J. Kneeland and W. Bernstein for sample collections, and the crew of the M/V Dream Island. We thank K. Selph of the UH SOEST flow cytometry facility for cell enumeration, J. Jennings of Oregon State University for inorganic nutrient analysis, and C. Wright and the University of Illinois W. M. Keck Center for Comparative and Functional Genomics for sequencing. The authors acknowledge the faculty of the 2011 MBL hosted STAMPS course for insight into sequencing data analysis and two insightful reviewers for their comments on this manuscript. This research was supported by a WHOI Ocean Life Institute postdoctoral scholar fellowship to A. Apprill, and by Award No. USA 00002 to K. Hughen made by King Abdullah University of Science and Technology (KAUST).
CollectionsPublications Acknowledging KAUST Support
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- Authors: Lampert Y, Kelman D, Dubinsky Z, Nitzan Y, Hill RT
- Issue date: 2006 Oct
- Successive shifts in the microbial community of the surface mucus layer and tissues of the coral Acropora muricata under thermal stress.
- Authors: Lee ST, Davy SK, Tang SL, Fan TY, Kench PS
- Issue date: 2015 Dec
- The Vibrio core group induces yellow band disease in Caribbean and Indo-Pacific reef-building corals.
- Authors: Cervino JM, Thompson FL, Gomez-Gil B, Lorence EA, Goreau TJ, Hayes RL, Winiarski-Cervino KB, Smith GW, Hughen K, Bartels E
- Issue date: 2008 Nov
- Phylogenetic diversity of bacteria associated with the mucus of Red Sea corals.
- Authors: Lampert Y, Kelman D, Nitzan Y, Dubinsky Z, Behar A, Hill RT
- Issue date: 2008 May
- Relationship of Vibrio species infection and elevated temperatures to yellow blotch/band disease in Caribbean corals.
- Authors: Cervino JM, Hayes RL, Polson SW, Polson SC, Goreau TJ, Martinez RJ, Smith GW
- Issue date: 2004 Nov