Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy
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AbstractMagnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.
CitationNappini S, Al Kayal T, Berti D, Nordèn B, Baglioni P (2011) Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy. The Journal of Physical Chemistry Letters 2: 713–718. Available: http://dx.doi.org/10.1021/jz2000936.
SponsorsFinancial support from CSGI, MIUR-PRIN (2008-20087K9A2J), and FIRB (RBPR05JH2P ITALNANONET) is acknowledged. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr. Tore Eriksson is acknowledged for building the alternating magnetic field generator.
PublisherAmerican Chemical Society (ACS)