Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin
Type
ArticleKAUST Grant Number
FIC/2010/07Date
2011-11-16Online Publication Date
2011-11-16Print Publication Date
2012-02-06Permanent link to this record
http://hdl.handle.net/10754/598689
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Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.Citation
Quitterer F, List A, Eisenreich W, Bacher A, Groll M (2011) Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin. Angew Chem 124: 1367–1370. Available: http://dx.doi.org/10.1002/ange.201106765.Sponsors
Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert.Publisher
WileyJournal
Angewandte Chemieae974a485f413a2113503eed53cd6c53
10.1002/ange.201106765