iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin
Type
ArticleAuthors
Han, ZhuangSun, Jin
Zhang, Yu
He, Fei
Xu, Ying
Matsumura, Kiyotaka
He, Li-Sheng
Qiu, Jian-Wen

Qi, Shu-Hua
Qian, Pei-Yuan

Date
2013-04-24Online Publication Date
2013-04-24Print Publication Date
2013-05-03Permanent link to this record
http://hdl.handle.net/10754/598679
Metadata
Show full item recordAbstract
Marine biofouling refers to the unwanted accumulation of fouling organisms, such as barnacles, on artificial surfaces, resulting in severe consequences for marine industries. Meleagrin is a potential nontoxic antifoulant that is isolated from the fungus Penicillium sp.; however, its mechanistic effect mode of action on larval settlement remains unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the effect of meleagrin on the proteomic expression profile of cyprid development and aging in the barnacle Balanus amphitrite. Fifty proteins were differentially expressed in response to treatment with meleagrin, among which 26 proteins were associated with cyprid development/aging and 24 were specifically associated with the meleagrin treatment. The 66 proteins that were associated with aging only remained unaltered during exposure to meleagrin. Using KEGG analysis, those proteins were assigned to several groups, including metabolic pathways, ECM-receptor interactions, and the regulation of the actin cytoskeleton. Among the 24 proteins that were not related to the development/aging process, expression of the cyprid major protein (CMP), a vitellogenin-like protein, increased after the meleagrin treatment, which suggested that meleagrin might affect the endocrine system and prevent the larval molting cycle. With the exception of the chitin binding protein that mediates the molting process and ATPase-mediated energy processes, the majority of proteins with significant effects in previous studies in response to cyprid treatment with butenolide and polyether B remained unchanged in the present study, suggesting that meleagrin may exhibit a different mechanism. © 2013 American Chemical Society.Citation
Han Z, Sun J, Zhang Y, He F, Xu Y, et al. (2013) iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin . Journal of Proteome Research 12: 2090–2100. Available: http://dx.doi.org/10.1021/pr301083e.Sponsors
This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.Publisher
American Chemical Society (ACS)Journal
Journal of Proteome ResearchPubMed ID
23540395ae974a485f413a2113503eed53cd6c53
10.1021/pr301083e
Scopus Count
Collections
Publications Acknowledging KAUST SupportRelated articles
- Comparative proteome and phosphoproteome analyses during cyprid development of the barnacle Balanus (=Amphibalanus) amphitrite.
- Authors: Zhang Y, Xu Y, Arellano SM, Xiao K, Qian PY
- Issue date: 2010 Jun 4
- Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids.
- Authors: Dash S, Chandramouli KH, Zhang Y, Qian PY
- Issue date: 2012
- Proteomic analysis of larvae during development, attachment, and metamorphosis in the fouling barnacle, Balanus amphitrite.
- Authors: Thiyagarajan V, Qian PY
- Issue date: 2008 Aug
- Modulation of barnacle (Balanus amphitrite Darwin) cyprid settlement behavior by sulfobetaine and carboxybetaine methacrylate polymer coatings.
- Authors: Aldred N, Li G, Gao Y, Clare AS, Jiang S
- Issue date: 2010 Aug
- The effect of butenolide on behavioral and morphological changes in two marine fouling species, the barnacle Balanus amphitrite and the bryozoan Bugula neritina.
- Authors: Zhang YF, Wang GC, Ying X, Sougrat R, Qian PY
- Issue date: 2011 May