iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin
Online Publication Date2013-04-24
Print Publication Date2013-05-03
Permanent link to this recordhttp://hdl.handle.net/10754/598679
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AbstractMarine biofouling refers to the unwanted accumulation of fouling organisms, such as barnacles, on artificial surfaces, resulting in severe consequences for marine industries. Meleagrin is a potential nontoxic antifoulant that is isolated from the fungus Penicillium sp.; however, its mechanistic effect mode of action on larval settlement remains unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the effect of meleagrin on the proteomic expression profile of cyprid development and aging in the barnacle Balanus amphitrite. Fifty proteins were differentially expressed in response to treatment with meleagrin, among which 26 proteins were associated with cyprid development/aging and 24 were specifically associated with the meleagrin treatment. The 66 proteins that were associated with aging only remained unaltered during exposure to meleagrin. Using KEGG analysis, those proteins were assigned to several groups, including metabolic pathways, ECM-receptor interactions, and the regulation of the actin cytoskeleton. Among the 24 proteins that were not related to the development/aging process, expression of the cyprid major protein (CMP), a vitellogenin-like protein, increased after the meleagrin treatment, which suggested that meleagrin might affect the endocrine system and prevent the larval molting cycle. With the exception of the chitin binding protein that mediates the molting process and ATPase-mediated energy processes, the majority of proteins with significant effects in previous studies in response to cyprid treatment with butenolide and polyether B remained unchanged in the present study, suggesting that meleagrin may exhibit a different mechanism. © 2013 American Chemical Society.
CitationHan Z, Sun J, Zhang Y, He F, Xu Y, et al. (2013) iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin . Journal of Proteome Research 12: 2090–2100. Available: http://dx.doi.org/10.1021/pr301083e.
SponsorsThis work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.
PublisherAmerican Chemical Society (ACS)
JournalJournal of Proteome Research
CollectionsPublications Acknowledging KAUST Support
- Comparative proteome and phosphoproteome analyses during cyprid development of the barnacle Balanus (=Amphibalanus) amphitrite.
- Authors: Zhang Y, Xu Y, Arellano SM, Xiao K, Qian PY
- Issue date: 2010 Jun 4
- Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids.
- Authors: Dash S, Chandramouli KH, Zhang Y, Qian PY
- Issue date: 2012
- Proteomic analysis of larvae during development, attachment, and metamorphosis in the fouling barnacle, Balanus amphitrite.
- Authors: Thiyagarajan V, Qian PY
- Issue date: 2008 Aug
- Butenolide inhibits marine fouling by altering the primary metabolism of three target organisms.
- Authors: Zhang YF, Zhang H, He L, Liu C, Xu Y, Qian PY
- Issue date: 2012 Jun 15
- Modulation of barnacle (Balanus amphitrite Darwin) cyprid settlement behavior by sulfobetaine and carboxybetaine methacrylate polymer coatings.
- Authors: Aldred N, Li G, Gao Y, Clare AS, Jiang S
- Issue date: 2010 Aug