Investigation of the binding of dioxin selective pentapeptides to a polyaniline matrix
Abstract
Polyaniline in form of emeraldine salt and emeraldine base was used as a matrix to attach several labeled and non-labeled dioxin selective pentapeptides both directly to the polymer and using glutaraldehyde as a linker. The peptides have been selected as a model to study the binding process due to their smaller size, lower sensitivity to the environment and potential application as solid state extraction reagents for chlorinated toxins. The composition and the properties of the compounds were investigated by means of elemental analysis, XPS, FTIR, UV/vis, and fluorescence spectroscopy. The results have shown that 3.30-7.76% peptides were attached to the emeraldine base both with and without a linker. Glutaraldehyde and the peptides were connected to the matrix via chemical bond resulting in formation of compounds whit similar composition and stability in a broad pH range. The influence of the linker and the peptides on the electronic properties and composition of the polymer have been investigated by principal component analysis. © 2012 Elsevier B.V. All rights reserved.Citation
Archibong E, Wang L, Ivanov I, Lita A, Redda K, et al. (2012) Investigation of the binding of dioxin selective pentapeptides to a polyaniline matrix. Synthetic Metals 162: 1255–1263. Available: http://dx.doi.org/10.1016/j.synthmet.2012.04.012.Sponsors
The material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2007-ST-061-000003. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security.The authors thank the National Institutes of Health, the National Institute of General Medical Sciences, MBRS Program (GM 08111) and Research Center at Minority Institutions Grant (RCMI) RR 03020.This publication was developed under an appointment to the DHS Summer Research Team Program for Minority Serving Institutions, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and U.S. Department of Homeland Security (DHS). ORISE is managed by Oak Ridge Associated Universities (ORAU) under DOE contract number DE-AC05-06OR23100. It has not been formally reviewed by DHS. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of DHS, DOE, or ORAU/ORISE. DHS, DOE and ORAU/ORISE do not endorse any products or commercial services mentioned in this publication.This work was supported in part by the MRSEC Program of the National Science Foundation under Award Number DMR-0819885, and also in part on work supported by Award No. KUS-C1-016-04, made by King Abdullah University of Science and Technology (KAUST).Publisher
Elsevier BVJournal
Synthetic MetalsISSN
0379-6779Handle URI
http://hdl.handle.net/10754/598668ae974a485f413a2113503eed53cd6c53
10.1016/j.synthmet.2012.04.012