Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement
Online Publication Date2012-08-10
Print Publication Date2012-08-21
Permanent link to this recordhttp://hdl.handle.net/10754/598642
MetadataShow full item record
AbstractInteractions between rotavirus and Suwannee River natural organic matter (NOM) were studied by time-resolved dynamic light scattering, quartz crystal microbalance, and atomic force microscopy. In NOM-containing NaCl solutions of up to 600 mM, rotavirus suspension remained stable for over 4 h. Atomic force microscopy (AFM) measurement for interaction force decay length at different ionic strengths showed that nonelectrostatic repulsive forces were mainly responsible for eliminating aggregation in NaCl solutions. Aggregation rates of rotavirus in solutions containing 20 mg C/L increased with divalent cation concentration until reaching a critical coagulation concentration of 30 mM CaCl2 or 70 mM MgCl2. Deposition kinetics of rotavirus on NOM-coated silica surface was studied using quartz crystal microbalance. Experimental attachment efficiencies for rotavirus adsorption to NOM-coated surface in MgCl2 solution were lower than in CaCl2 solution at a given divalent cation concentration. Stronger adhesion force was measured for virus-virus and virus-NOM interactions in CaCl2 solution compared to those in MgCl2 or NaCl solutions at the same ionic strength. This study suggested that divalent cation complexation with carboxylate groups in NOM and on virus surface was an important mechanism in the deposition and aggregation kinetics of rotavirus. © 2012 American Chemical Society.
CitationGutierrez L, Nguyen TH (2012) Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement. Environ Sci Technol 46: 8705–8713. Available: http://dx.doi.org/10.1021/es301336u.
SponsorsThis work was partially supported by NSF #0954501, the Academic Excellence Alliance program at King Abdullah University of Science and Technology, and the U.S. Department of Energy DE-FG02-07ER46453 and DE-FG02-07ER46471. We also acknowledge Dr. Scott McLaren, Ms. Ofelia Romero, and Mr. Tony Straub.
PublisherAmerican Chemical Society (ACS)
CollectionsPublications Acknowledging KAUST Support
- Deposition and aggregation kinetics of rotavirus in divalent cation solutions.
- Authors: Gutierrez L, Mylon SE, Nash B, Nguyen TH
- Issue date: 2010 Jun 15
- Interactions between rotavirus and natural organic matter isolates with different physicochemical characteristics.
- Authors: Gutierrez L, Nguyen TH
- Issue date: 2013 Nov 26
- Deposition kinetics of zinc oxide nanoparticles on natural organic matter coated silica surfaces.
- Authors: Jiang X, Tong M, Li H, Yang K
- Issue date: 2010 Oct 15
- Coating of AFM probes with aquatic humic and non-humic NOM to study their adhesion properties.
- Authors: Aubry C, Gutierrez L, Croue JP
- Issue date: 2013 Jun 1
- Deposition kinetics of bacteriophage MS2 to natural organic matter: role of divalent cations.
- Authors: Pham M, Mintz EA, Nguyen TH
- Issue date: 2009 Oct 1