Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots
Pound, M. P.
Byrne, H. M.
Hodgman, T. C.
King, J. R.
Pridmore, T. P.
Bennett, M. J.
KAUST Grant NumberKUK-013-04
Permanent link to this recordhttp://hdl.handle.net/10754/598640
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AbstractAs multicellular organisms grow, positional information is continually needed to regulate the pattern in which cells are arranged. In the Arabidopsis root, most cell types are organized in a radially symmetric pattern; however, a symmetry-breaking event generates bisymmetric auxin and cytokinin signaling domains in the stele. Bidirectional cross-talk between the stele and the surrounding tissues involving a mobile transcription factor, SHORT ROOT (SHR), and mobile microRNA species also determines vascular pattern, but it is currently unclear how these signals integrate. We use a multicellular model to determine a minimal set of components necessary for maintaining a stable vascular pattern. Simulations perturbing the signaling network show that, in addition to the mutually inhibitory interaction between auxin and cytokinin, signaling through SHR, microRNA165/6, and PHABULOSA is required to maintain a stable bisymmetric pattern. We have verified this prediction by observing loss of bisymmetry in shr mutants. The model reveals the importance of several features of the network, namely the mutual degradation of microRNA165/6 and PHABULOSA and the existence of an additional negative regulator of cytokinin signaling. These components form a plausible mechanism capable of patterning vascular tissues in the absence of positional inputs provided by the transport of hormones from the shoot.
CitationMuraro D, Mellor N, Pound MP, Help H, Lucas M, et al. (2013) Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots. Proceedings of the National Academy of Sciences 111: 857–862. Available: http://dx.doi.org/10.1073/pnas.1221766111.
SponsorsWe thank Leah Band for helpful comments. We acknowledge the Biotechnology and Biological Sciences Research Council and the Engineering and Physical Sciences Research Council for funding. H. M. B. was funded, in part, by King Abdullah University of Science and Technology (KAUST) Award KUK-013-04, J.R.K. was funded by the Royal Society and Wolfson Foundation, and A. B. was funded by a Royal Society University Research Fellowship. J.G and C. G. were funded by the Institut de Biologie Computationnelle de Montpellier and the Morphogenetics Inria Project-Lab.
PubMed Central IDPMC3896157
CollectionsPublications Acknowledging KAUST Support
- A mutually inhibitory interaction between auxin and cytokinin specifies vascular pattern in roots.
- Authors: Bishopp A, Help H, El-Showk S, Weijers D, Scheres B, Friml J, Benková E, Mähönen AP, Helariutta Y
- Issue date: 2011 Jun 7
- North, East, South, West: mapping vascular tissues onto the Arabidopsis root.
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- Issue date: 2018 Feb
- Parsimonious Model of Vascular Patterning Links Transverse Hormone Fluxes to Lateral Root Initiation: Auxin Leads the Way, while Cytokinin Levels Out.
- Authors: el-Showk S, Help-Rinta-Rahko H, Blomster T, Siligato R, Marée AF, Mähönen AP, Grieneisen VA
- Issue date: 2015 Oct
- Genome-wide direct target analysis reveals a role for SHORT-ROOT in root vascular patterning through cytokinin homeostasis.
- Authors: Cui H, Hao Y, Kovtun M, Stolc V, Deng XW, Sakakibara H, Kojima M
- Issue date: 2011 Nov
- The transcription factors BEL1 and SPL are required for cytokinin and auxin signaling during ovule development in Arabidopsis.
- Authors: Bencivenga S, Simonini S, Benková E, Colombo L
- Issue date: 2012 Jul