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dc.contributor.authorKogan, Maxim
dc.contributor.authorNordén, Bengt
dc.contributor.authorBeke-Somfai, Tamás
dc.date.accessioned2016-02-25T13:21:09Z
dc.date.available2016-02-25T13:21:09Z
dc.date.issued2013-10
dc.identifier.citationKogan M, Nordén B, Beke-Somfai T (2013) High anisotropy of flow-aligned bicellar membrane systems. Chemistry and Physics of Lipids 175-176: 105–115. Available: http://dx.doi.org/10.1016/j.chemphyslip.2013.08.006.
dc.identifier.issn0009-3084
dc.identifier.pmid23999012
dc.identifier.doi10.1016/j.chemphyslip.2013.08.006
dc.identifier.urihttp://hdl.handle.net/10754/598463
dc.description.abstractIn recent years, multi-lipid bicellar systems have emerged as promising membrane models. The fast orientational diffusion and magnetic alignability made these systems very attractive for NMR investigations. However, their alignment was so far achieved with a strong magnetic field, which limited their use with other methods that require macroscopic orientation. Recently, it was shown that bicelles could be aligned also by shear flow in a Couette flow cell, making it applicable to structural and biophysical studies by polarized light spectroscopy. Considering the sensitivity of this lipid system to small variations in composition and physicochemical parameters, efficient use of such a flow-cell method with coupled techniques will critically depend on the detailed understanding of how the lipid systems behave under flow conditions. In the present study we have characterized the flow alignment behavior of the commonly used dimyristoyl phosphatidylcholine/dicaproyl phosphatidylcholine (DMPC/DHPC) bicelle system, for various temperatures, lipid compositions, and lipid concentrations. We conclude that at optimal flow conditions the selected bicellar systems can produce the most efficient flow alignment out of any lipid systems used so far. The highest degree of orientation of DMPC/DHPC samples is noticed in a narrow temperature interval, at a practical temperature around 25 C, most likely in the phase transition region characterized by maximum sample viscosity. The change of macroscopic orientation factor as function of the above conditions is now described in detail. The increase in macroscopic alignment observed for bicelles will most likely allow recording of higher resolution spectra on membrane systems, which provide deeper structural insight and analysis into properties of biomolecules interacting with solution phase lipid membranes. © 2013 Elsevier Ireland Ltd.
dc.description.sponsorshipKing Abdullah University of Science and Technology (KAUST) is gratefully acknowledged for financing this research (Grant KUK-11-008-23).
dc.publisherElsevier BV
dc.subjectAlignment
dc.subjectBicelle
dc.subjectFlow-orientation
dc.subjectLinear dichroism
dc.subjectLipid membrane
dc.subjectLiposome
dc.titleHigh anisotropy of flow-aligned bicellar membrane systems
dc.typeArticle
dc.identifier.journalChemistry and Physics of Lipids
dc.contributor.institutionChalmers University of Technology, Göteborg, Sweden
kaust.grant.numberKUK-11-008-23


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