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    High anisotropy of flow-aligned bicellar membrane systems

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    Type
    Article
    Authors
    Kogan, Maxim
    Nordén, Bengt
    Beke-Somfai, Tamás
    KAUST Grant Number
    KUK-11-008-23
    Date
    2013-10
    Permanent link to this record
    http://hdl.handle.net/10754/598463
    
    Metadata
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    Abstract
    In recent years, multi-lipid bicellar systems have emerged as promising membrane models. The fast orientational diffusion and magnetic alignability made these systems very attractive for NMR investigations. However, their alignment was so far achieved with a strong magnetic field, which limited their use with other methods that require macroscopic orientation. Recently, it was shown that bicelles could be aligned also by shear flow in a Couette flow cell, making it applicable to structural and biophysical studies by polarized light spectroscopy. Considering the sensitivity of this lipid system to small variations in composition and physicochemical parameters, efficient use of such a flow-cell method with coupled techniques will critically depend on the detailed understanding of how the lipid systems behave under flow conditions. In the present study we have characterized the flow alignment behavior of the commonly used dimyristoyl phosphatidylcholine/dicaproyl phosphatidylcholine (DMPC/DHPC) bicelle system, for various temperatures, lipid compositions, and lipid concentrations. We conclude that at optimal flow conditions the selected bicellar systems can produce the most efficient flow alignment out of any lipid systems used so far. The highest degree of orientation of DMPC/DHPC samples is noticed in a narrow temperature interval, at a practical temperature around 25 C, most likely in the phase transition region characterized by maximum sample viscosity. The change of macroscopic orientation factor as function of the above conditions is now described in detail. The increase in macroscopic alignment observed for bicelles will most likely allow recording of higher resolution spectra on membrane systems, which provide deeper structural insight and analysis into properties of biomolecules interacting with solution phase lipid membranes. © 2013 Elsevier Ireland Ltd.
    Citation
    Kogan M, Nordén B, Beke-Somfai T (2013) High anisotropy of flow-aligned bicellar membrane systems. Chemistry and Physics of Lipids 175-176: 105–115. Available: http://dx.doi.org/10.1016/j.chemphyslip.2013.08.006.
    Sponsors
    King Abdullah University of Science and Technology (KAUST) is gratefully acknowledged for financing this research (Grant KUK-11-008-23).
    Publisher
    Elsevier BV
    Journal
    Chemistry and Physics of Lipids
    DOI
    10.1016/j.chemphyslip.2013.08.006
    PubMed ID
    23999012
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.chemphyslip.2013.08.006
    Scopus Count
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