Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical systems
KAUST Grant NumberKUS-I1-003-13
Online Publication Date2014-07-22
Print Publication Date2014-10-01
Permanent link to this recordhttp://hdl.handle.net/10754/598407
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Abstract© 2014 IUMS. A previously isolated exoelectrogenic bacterium, strain SD-1(T), was further characterized and identified as a representative of a novel species of the genus Geobacter. Strain SD-1(T) was Gram-negative, aerotolerant, anaerobic, non-spore-forming, non-fermentative and non-motile. Cells were short, curved rods (0.8-1.3 µm long and 0.3 µm in diameter). Growth of strain SD-1(T) was observed at 15-42 °C and pH 6.0-8.5, with optimal growth at 30-35 °C and pH 7. Analysis of 16S rRNA gene sequences indicated that the isolate was a member of the genus Geobacter, with the closest known relative being Geobacter sulfurreducens PCA(T) (98% similarity). Similar to other members of the genus Geobacter, strain SD-1(T) used soluble or insoluble Fe(III) as the sole electron acceptor coupled with the oxidation of acetate. However, SD-1(T) could not reduce fumarate as an electron acceptor with acetate oxidization, which is an important physiological trait for G. sulfurreducens. Moreover, SD-1(T) could grow in media containing as much as 3% NaCl, while G. sulfurreducens PCA(T) can tolerate just half this concentration, and this difference in salt tolerance was even more obvious when cultivated in bioelectrochemical systems. DNA-DNA hybridization analysis of strain SD-1(T) and its closest relative, G. sulfurreducens ATCC 51573(T), showed a relatedness of 61.6%. The DNA G+C content of strain SD-1(T) was 58.9 mol%. Thus, on the basis of these characteristics, strain SD-1(T) was not assigned to G. sulfurreducens, and was instead classified in the genus Geobacter as a representative of a novel species. The name Geobacter anodireducens sp. nov. is proposed, with the type strain SD-1(T) ( = CGMCC 1.12536(T) = KCTC 4672(T)).
CitationSun D, Wang A, Cheng S, Yates M, Logan BE (2014) Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical systems. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64: 3485–3491. Available: http://dx.doi.org/10.1099/ijs.0.061598-0.
SponsorsWe thank the China General Microbiological Culture Collection Center for their help with DNA DNA hybridization and DNA G + C content analysis. This research was supported by the King Abdullah University of Science and Technology (KAUST) (award KUS-I1-003-13), the China Postdoctoral Science Foundation (grant nos 2013M541773 and 2014T70573), the National Science Foundation for Distinguished Young Scholars (grant no. 51225802) and the Science Fund for Creative Research Groups of the National Natural Science Foundation of China (grant no. 51121062).
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