Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light
KAUST Grant NumberKUK-11-008-23
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AbstractBicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.
CitationKogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.
SponsorsThis work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).
PublisherRoyal Society of Chemistry (RSC)
CollectionsPublications Acknowledging KAUST Support
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