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    Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

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    Type
    Article
    Authors
    Kogan, Maxim
    Beke-Somfai, Tamás
    Nordén, Bengt
    KAUST Grant Number
    KUK-11-008-23
    Date
    2011
    Permanent link to this record
    http://hdl.handle.net/10754/598344
    
    Metadata
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    Abstract
    Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.
    Citation
    Kogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.
    Sponsors
    This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).
    Publisher
    Royal Society of Chemistry (RSC)
    Journal
    Chemical Communications
    DOI
    10.1039/c1cc12313k
    PubMed ID
    21637893
    ae974a485f413a2113503eed53cd6c53
    10.1039/c1cc12313k
    Scopus Count
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