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    Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications

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    Type
    Article
    Authors
    Norville, Julie E.
    Kelly, Deborah F.
    Knight, Thomas F.
    Belcher, Angela M.
    Walz, Thomas
    Date
    2011-06-17
    Online Publication Date
    2011-06-17
    Print Publication Date
    2011-07
    Permanent link to this record
    http://hdl.handle.net/10754/598314
    
    Metadata
    Show full item record
    Abstract
    A goal of synthetic biology is to make biological systems easier to engineer. One of the aims is to design, with nanometer-scale precision, biomaterials with well-defined properties. The surface-layer protein SbpA forms 2D arrays naturally on the cell surface of Lysinibacillus sphaericus, but also as the purified protein in solution upon the addition of divalent cations. The high propensity of SbpA to form crystalline arrays, which can be simply controlled by divalent cations, and the possibility to genetically alter the protein, make SbpA an attractive molecule for synthetic biology. To be a useful tool, however, it is important that a simple protocol can be used to produce recombinant wild-type and modified SbpA in large quantities and in a biologically active form. The present study addresses this requirement by introducing a mild and non-denaturing purification protocol to produce milligram quantities of recombinant, active SbpA.
    Citation
    Norville JE, Kelly DF, Knight TF, Belcher AM, Walz T (2011) Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications. Biotechnology Journal 6: 807–811. Available: http://dx.doi.org/10.1002/biot.201100024.
    Sponsors
    This work was supported by a KAUST Scholar Graduate Research Fellowship (J.E.N.), the SynBERC NSF ERC (www.synberc.org) (J.E.N. and T. F. K.), National Institutes of Health grant GM52580 (S. C. Harrison) and the U.S. Army Research Office through both the Institute for Soldier Nanotechnologies and the Institute for Collaborative Biotechnologies (A. M. B.). T. W. is an investigator in the Howard Hughes Medical Institute.
    Publisher
    Wiley
    Journal
    Biotechnology Journal
    DOI
    10.1002/biot.201100024
    PubMed ID
    21681963
    PubMed Central ID
    PMC4374430
    ae974a485f413a2113503eed53cd6c53
    10.1002/biot.201100024
    Scopus Count
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