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dc.contributor.authorKirkham, Glen R.
dc.contributor.authorLovrics, Anna
dc.contributor.authorByrne, Helen M.
dc.contributor.authorJensen, Oliver E.
dc.contributor.authorKing, John R.
dc.contributor.authorShakesheff, Kevin M.
dc.contributor.authorButtery, Lee D. K.
dc.date.accessioned2016-02-25T13:11:37Z
dc.date.available2016-02-25T13:11:37Z
dc.date.issued2012
dc.identifier.citationKirkham GR, Lovrics A, Byrne HM, Jensen OE, King JR, et al. (2012) Early gene regulation of osteogenesis in embryonic stem cells. Integrative Biology 4: 1470. Available: http://dx.doi.org/10.1039/c2ib20164j.
dc.identifier.issn1757-9694
dc.identifier.issn1757-9708
dc.identifier.pmid23042286
dc.identifier.doi10.1039/c2ib20164j
dc.identifier.urihttp://hdl.handle.net/10754/598045
dc.description.abstractThe early gene regulatory networks (GRNs) that mediate stem cell differentiation are complex, and the underlying regulatory associations can be difficult to map accurately. In this study, the expression profiles of the genes Dlx5, Msx2 and Runx2 in mouse embryonic stem cells were monitored over a 48 hour period after exposure to the growth factors BMP2 and TGFβ1. Candidate GRNs of early osteogenesis were constructed based on published experimental findings and simulation results of Boolean and ordinary differential equation models were compared with our experimental data in order to test the validity of these models. Three gene regulatory networks were found to be consistent with the data, one of these networks exhibited sustained oscillation, a behaviour which is consistent with the general view of embryonic stem cell plasticity. The work cycle presented in this paper illustrates how mathematical modelling can be used to elucidate from gene expression profiles GRNs that are consistent with experimental data. © 2012 The Royal Society of Chemistry.
dc.description.sponsorshipThe BBSRC/EPSRC for funding project number BB/D008522/1. This publication was based on work supported in part by Award No. KUK-C1-013-03, made by King Abdullah University of Science and Technology (KAUST).
dc.publisherOxford University Press (OUP)
dc.titleEarly gene regulation of osteogenesis in embryonic stem cells
dc.typeArticle
dc.identifier.journalIntegrative Biology
dc.contributor.institutionCentre for Biomolecular Sciences, Nottingham, United Kingdom
dc.contributor.institutionUniversity of Nottingham, Nottingham, United Kingdom
dc.contributor.institutionBiotalentum Ltd., Godollo, Hungary
dc.contributor.institutionUniversity of Oxford, Oxford, United Kingdom
kaust.grant.numberKUK-C1-013-03


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