Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy
KAUST Grant NumberKUK-I1-007-43
Permanent link to this recordhttp://hdl.handle.net/10754/597956
MetadataShow full item record
AbstractFor the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.
CitationPunzet M, Baurecht D, Varga F, Karlic H, Heitzinger C (2012) Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy. Nanoscale 4: 2431. Available: http://dx.doi.org/10.1039/c2nr12038k.
SponsorsWe thank Prof. Wolfgang Lindner of the Chemical Faculty of the University of Vienna for his assistance in silane chemistry and the facilitation of the silanization protocol and Prof. Falkenhagen of the Danube University, Krems (Austria) for providing us with monoclonal hTNF-alpha antibody. This work has been financially supported by Austrian Science Fund (FWF) Project No. P20871-N31 and by Award No. KUK-I1-007-43, made by the King Abdullah University of Science and Technology (KAUST). We dedicate this article to the memory of Prof. Urs Peter Fringeli.
PublisherRoyal Society of Chemistry (RSC)
CollectionsPublications Acknowledging KAUST Support
- Chemical sensing with nanowires.
- Authors: Penner RM
- Issue date: 2012
- Sensing of protein adsorption with a porous bulk composite comprising silver nanoparticles deposited on hydroxyapatite.
- Authors: Ohtsuki C, Ichikawa Y, Shibata H, Kawachi G, Torimoto T, Ogata S
- Issue date: 2010 Apr
- In situ FTIR ATR spectroscopic study of the interaction of immobilized human tumor necrosis factor-alpha with a monoclonal antibody in aqueous environment.
- Authors: Reiter G, Hassler N, Weber V, Falkenhagen D, Fringeli UP
- Issue date: 2004 Jun 1
- Ultrasensitive protein detection using lithographically defined Si multi-nanowire field effect transistors.
- Authors: Tian R, Regonda S, Gao J, Liu Y, Hu W
- Issue date: 2011 Jun 7
- Nanoparticle-assisted micropatterning of active proteins on solid substrate.
- Authors: Wang C, Zhang Y, Seng HS, Ngo LL
- Issue date: 2006 Feb 15