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    Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates

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    Type
    Article
    Authors
    Lewis, Jared C.
    Mantovani, Simone M.
    Fu, Yu
    Snow, Christopher D.
    Komor, Russell S.
    Wong , Chi-Huey
    Arnold, Frances H.
    KAUST Grant Number
    KUS-F1-028-03
    Date
    2010-11-24
    Online Publication Date
    2010-11-24
    Print Publication Date
    2010-12-10
    Permanent link to this record
    http://hdl.handle.net/10754/597800
    
    Metadata
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    Abstract
    Made for each other: Combinatorial alanine substitution of active site residues in a thermostable cytochrome P450BM3 variant was used to generate an enzyme that is active with large substrates. Selective hydroxylation of methoxymethylated monosaccharides, alkaloids, and steroids was thus made possible (see Scheme). This approach could be useful for improving the activity of enzymes that show only limited activity with larger substrates. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Citation
    Lewis JC, Mantovani SM, Fu Y, Snow CD, Komor RS, et al. (2010) Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates. ChemBioChem 11: 2502–2505. Available: http://dx.doi.org/10.1002/cbic.201000565.
    Sponsors
    J.C.L. is supported by a U.S. National Institutes of Health Pathways to Independence Award (1K99M087551-01A1). S.M.M. is supported by the Fundacao Coordenadoria de Aperfeicoamento de Pessoal de Nivel Superior (CAPES; 1756-09-5). This work was supported by the U.S. National Institutes of Health (2R01 M068664-05A1), the U.S. Department of Energy, Office of Basic Science, grant DE-FG02-06ER15762, and King Abdullah University of Science and Technology (KAUST), Award No. KUS-F1-028-03.
    Publisher
    Wiley
    Journal
    ChemBioChem
    DOI
    10.1002/cbic.201000565
    PubMed ID
    21108271
    PubMed Central ID
    PMC4447097
    ae974a485f413a2113503eed53cd6c53
    10.1002/cbic.201000565
    Scopus Count
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