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dc.contributor.authorRose, Nicholas D.
dc.contributor.authorRegan, John M.
dc.date.accessioned2016-02-25T12:56:04Z
dc.date.available2016-02-25T12:56:04Z
dc.date.issued2015-12
dc.identifier.citationRose ND, Regan JM (2015) Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation. Bioelectrochemistry 106: 213–220. Available: http://dx.doi.org/10.1016/j.bioelechem.2015.03.003.
dc.identifier.issn1567-5394
dc.identifier.pmid25857596
dc.identifier.doi10.1016/j.bioelechem.2015.03.003
dc.identifier.urihttp://hdl.handle.net/10754/597751
dc.description.abstract© 2015 Elsevier B.V. Geobacter sulfurreducens is one of the dominant bacterial species found in biofilms growing on anodes in bioelectrochemical systems. The intracellular concentrations of reduced and oxidized forms of nicotinamide-adenine dinucleotide (NADH and NAD$^{+}$, respectively) and nicotinamide-adenine dinucleotide phosphate (NADPH and NADP$^{+}$, respectively) as well as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were measured in G. sulfurreducens using fumarate, Fe(III)-citrate, or anodes poised at different potentials (110, 10, -90, and -190mV (vs. SHE)) as the electron acceptor. The ratios of CNADH/CNAD+ (0.088±0.022) and CNADPH/CNADP+ (0.268±0.098) were similar under all anode potentials tested and with Fe(III)-citrate (reduced extracellularly). Both ratios significantly increased with fumarate as the electron acceptor (0.331±0.094 for NAD and 1.96±0.37 for NADP). The adenylate energy charge (the fraction of phosphorylation in intracellular adenosine phosphates) was maintained near 0.47 under almost all conditions. Anode-growing biofilms demonstrated a significantly higher molar ratio of ATP/ADP relative to suspended cultures grown on fumarate or Fe(III)-citrate. These results provide evidence that the cellular location of reduction and not the redox potential of the electron acceptor controls the intracellular redox potential in G. sulfurreducens and that biofilm growth alters adenylate phosphorylation.
dc.description.sponsorshipThis research was supported by Award KUS-I1-003-13 from the KingAbdullah University of Science and Technology (KAUST).
dc.publisherElsevier BV
dc.subjectAdenosine phosphate
dc.subjectGeobacter sulfurreducens
dc.subjectNicotinamide adenine dinucleotide
dc.subjectRedox potential
dc.titleChanges in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation
dc.typeArticle
dc.identifier.journalBioelectrochemistry
dc.contributor.institutionPennsylvania State University, State College, United States
dc.contributor.institutionInc., Boonton, United States
kaust.grant.numberKUS-I1-003-13


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