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    Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation

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    Type
    Article
    Authors
    Rose, Nicholas D.
    Regan, John M.
    KAUST Grant Number
    KUS-I1-003-13
    Date
    2015-12
    Permanent link to this record
    http://hdl.handle.net/10754/597751
    
    Metadata
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    Abstract
    © 2015 Elsevier B.V. Geobacter sulfurreducens is one of the dominant bacterial species found in biofilms growing on anodes in bioelectrochemical systems. The intracellular concentrations of reduced and oxidized forms of nicotinamide-adenine dinucleotide (NADH and NAD$^{+}$, respectively) and nicotinamide-adenine dinucleotide phosphate (NADPH and NADP$^{+}$, respectively) as well as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were measured in G. sulfurreducens using fumarate, Fe(III)-citrate, or anodes poised at different potentials (110, 10, -90, and -190mV (vs. SHE)) as the electron acceptor. The ratios of CNADH/CNAD+ (0.088±0.022) and CNADPH/CNADP+ (0.268±0.098) were similar under all anode potentials tested and with Fe(III)-citrate (reduced extracellularly). Both ratios significantly increased with fumarate as the electron acceptor (0.331±0.094 for NAD and 1.96±0.37 for NADP). The adenylate energy charge (the fraction of phosphorylation in intracellular adenosine phosphates) was maintained near 0.47 under almost all conditions. Anode-growing biofilms demonstrated a significantly higher molar ratio of ATP/ADP relative to suspended cultures grown on fumarate or Fe(III)-citrate. These results provide evidence that the cellular location of reduction and not the redox potential of the electron acceptor controls the intracellular redox potential in G. sulfurreducens and that biofilm growth alters adenylate phosphorylation.
    Citation
    Rose ND, Regan JM (2015) Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation. Bioelectrochemistry 106: 213–220. Available: http://dx.doi.org/10.1016/j.bioelechem.2015.03.003.
    Sponsors
    This research was supported by Award KUS-I1-003-13 from the KingAbdullah University of Science and Technology (KAUST).
    Publisher
    Elsevier BV
    Journal
    Bioelectrochemistry
    DOI
    10.1016/j.bioelechem.2015.03.003
    PubMed ID
    25857596
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bioelechem.2015.03.003
    Scopus Count
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