Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation
KAUST Grant NumberKUS-I1-003-13
MetadataShow full item record
Abstract© 2015 Elsevier B.V. Geobacter sulfurreducens is one of the dominant bacterial species found in biofilms growing on anodes in bioelectrochemical systems. The intracellular concentrations of reduced and oxidized forms of nicotinamide-adenine dinucleotide (NADH and NAD<sup>+</sup>, respectively) and nicotinamide-adenine dinucleotide phosphate (NADPH and NADP<sup>+</sup>, respectively) as well as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were measured in G. sulfurreducens using fumarate, Fe(III)-citrate, or anodes poised at different potentials (110, 10, -90, and -190mV (vs. SHE)) as the electron acceptor. The ratios of CNADH/CNAD+ (0.088±0.022) and CNADPH/CNADP+ (0.268±0.098) were similar under all anode potentials tested and with Fe(III)-citrate (reduced extracellularly). Both ratios significantly increased with fumarate as the electron acceptor (0.331±0.094 for NAD and 1.96±0.37 for NADP). The adenylate energy charge (the fraction of phosphorylation in intracellular adenosine phosphates) was maintained near 0.47 under almost all conditions. Anode-growing biofilms demonstrated a significantly higher molar ratio of ATP/ADP relative to suspended cultures grown on fumarate or Fe(III)-citrate. These results provide evidence that the cellular location of reduction and not the redox potential of the electron acceptor controls the intracellular redox potential in G. sulfurreducens and that biofilm growth alters adenylate phosphorylation.
CitationRose ND, Regan JM (2015) Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation. Bioelectrochemistry 106: 213–220. Available: http://dx.doi.org/10.1016/j.bioelechem.2015.03.003.
SponsorsThis research was supported by Award KUS-I1-003-13 from the KingAbdullah University of Science and Technology (KAUST).
CollectionsPublications Acknowledging KAUST Support
- Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides.
- Authors: Chan CH, Levar CE, Jiménez-Otero F, Bond DR
- Issue date: 2017 Oct 1
- Growth of Geobacter sulfurreducens under nutrient-limiting conditions in continuous culture.
- Authors: Esteve-Núñez A, Rothermich M, Sharma M, Lovley D
- Issue date: 2005 May
- Anode biofilm transcriptomics reveals outer surface components essential for high density current production in Geobacter sulfurreducens fuel cells.
- Authors: Nevin KP, Kim BC, Glaven RH, Johnson JP, Woodard TL, Methé BA, Didonato RJ, Covalla SF, Franks AE, Liu A, Lovley DR
- Issue date: 2009 May 20
- Spatially resolved confocal resonant Raman microscopic analysis of anode-grown Geobacter sulfurreducens biofilms.
- Authors: Lebedev N, Strycharz-Glaven SM, Tender LM
- Issue date: 2014 Feb 3
- Aromatic amino acids required for pili conductivity and long-range extracellular electron transport in Geobacter sulfurreducens.
- Authors: Vargas M, Malvankar NS, Tremblay PL, Leang C, Smith JA, Patel P, Snoeyenbos-West O, Nevin KP, Lovley DR
- Issue date: 2013 Mar 12