Change in microbial communities in acetate- and glucose-fed microbial fuel cells in the presence of light

Type
Article

Authors
Xing, Defeng
Cheng, Shaoan
Regan, John M.
Logan, Bruce E.

KAUST Grant Number
KUS-11-003-13

Date
2009-09

Abstract
Power densities produced by microbial fuel cells (MFCs) in natural systems are changed by exposure to light through the enrichment of photosynthetic microorganisms. When MFCs with brush anodes were exposed to light (4000 lx), power densities increased by 8-10% for glucose-fed reactors, and 34% for acetate-fed reactors. Denaturing gradient gel electrophoresis (DGGE) profiles based on the 16S rRNA gene showed that exposure to high light levels changed the microbial communities on the anodes. Based on 16S rRNA gene clone libraries of light-exposed systems the anode communities using glucose were also significantly different than those fed acetate. Dominant bacteria that are known exoelectrogens were identified in the anode biofilm, including a purple nonsulfur (PNS) photosynthetic bacterium, Rhodopseudomonas palustris, and a dissimilatory iron-reducing bacterium, Geobacter sulfurreducens. Pure culture tests confirmed that PNS photosynthetic bacteria increased power production when exposed to high light intensities (4000 lx). These results demonstrate that power production and community composition are affected by light conditions as well as electron donors in single-chamber air-cathode MFCs. © 2009 Elsevier B.V. All rights reserved.

Citation
Xing D, Cheng S, Regan JM, Logan BE (2009) Change in microbial communities in acetate- and glucose-fed microbial fuel cells in the presence of light. Biosensors and Bioelectronics 25: 105–111. Available: http://dx.doi.org/10.1016/j.bios.2009.06.013.

Acknowledgements
This research was funded by a grant from the U.S. Air Force Office of Scientific Research, and Award KUS-11-003-13 by King Abdullah University of Science and Technology (KAUST).

Publisher
Elsevier BV

Journal
Biosensors and Bioelectronics

DOI
10.1016/j.bios.2009.06.013

PubMed ID
19574034

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