Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation
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AbstractWe present a ratio controlled cell-to-cell interaction chip using valve-less isolation. We incorporated electrolysis in a microfluidic channel. In each microfluidic chamber, we loaded two types of different cells at various pairing ratios. More than 80% of the microchambers were successfully loaded with a specific target pairing ratio. For the proof of concept, we have demonstrated the cell-to-cell interaction between prostate cancer cells and muscle stem cells can be controlled by cell pairing ratios through growth factor secretion. The experimental data shows that sealing of microenvironment by air generated from electrolysis does not affect cell viability and cell interaction assay results. © 2012 IEEE.
CitationChen Y-C, Lou X, Ingram P, Yoon E (2012) Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation. 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS). Available: http://dx.doi.org/10.1109/MEMSYS.2012.6170305.
SponsorsThis work was supported in part by the Thermo Fisher Scientific Screening Technology Grant under the Center for Chemical Genomics at the Life Sciences Institute at the University of Michigan, and in part by Academic Excellence Alliance Award from KAUST. The cells are provided by Prof. Buckanovich at the Comprehensive Cancer Center at the University of Michigan.