Antibody structural modeling with prediction of immunoglobulin structure (PIGS)

Type
Article

Authors
Marcatili, Paolo
Olimpieri, Pier Paolo
Chailyan, Anna
Tramontano, Anna

Online Publication Date
2014-11-06

Print Publication Date
2014-12

Date
2014-11-06

Abstract
© 2014 Nature America, Inc. All rights reserved. Antibodies (or immunoglobulins) are crucial for defending organisms from pathogens, but they are also key players in many medical, diagnostic and biotechnological applications. The ability to predict their structure and the specific residues involved in antigen recognition has several useful applications in all of these areas. Over the years, we have developed or collaborated in developing a strategy that enables researchers to predict the 3D structure of antibodies with a very satisfactory accuracy. The strategy is completely automated and extremely fast, requiring only a few minutes (~10 min on average) to build a structural model of an antibody. It is based on the concept of canonical structures of antibody loops and on our understanding of the way light and heavy chains pack together.

Citation
Marcatili P, Olimpieri PP, Chailyan A, Tramontano A (2014) Antibody structural modeling with prediction of immunoglobulin structure (PIGS). Nature Protocols 9: 2771–2783. Available: http://dx.doi.org/10.1038/nprot.2014.189.

Acknowledgements
The authors are grateful to all the members of the Sapienza University of Rome Biocomputing Group for their help and assistance. We are thankful to the King Abdullah University of Science and Technology (KAUST) for financial support.

Publisher
Springer Nature

Journal
Nature Protocols

DOI
10.1038/nprot.2014.189

PubMed ID
25375991

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