Analysis of plasma protein adsorption onto DC-Chol-DOPE cationic liposomes by HPLC-CHIP coupled to a Q-TOF mass spectrometer
AuthorsCapriotti, Anna Laura
KAUST Grant NumberKUK-F1-036-32
Permanent link to this recordhttp://hdl.handle.net/10754/597561
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AbstractPlasma protein adsorption is regarded as a key factor in the in vivo organ distribution of intravenously administered drug carriers, and strongly depends on vector surface characteristics. The present study aimed to characterize the "protein corona" absorbed onto DC-Chol-DOPE cationic liposomes. This system was chosen because it is one of the most efficient and widely used non-viral formulations in vitro and a potential candidate for in vivo transfection of genetic material. After incubation of human plasma with cationic liposomes, nanoparticle-protein complex was separated from plasma by centrifugation. An integrated approach based on protein separation by one-dimensional 12% polyacrylamide gel electrophoresis followed by the automated HPLC-Chip technology coupled to a high-resolution mass spectrometer was employed for protein corona characterization. Thirty gel lanes, approximately 2 mm, were cut, digested and analyzed by HPLC-MS/MS. Fifty-eight human plasma proteins adsorbed onto DC-Chol-DOPE cationic liposomes were identified. The knowledge of the interactions of proteins with liposomes can be exploited for future controlled design of colloidal drug carriers and possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins in body fluids. © 2010 Springer-Verlag.
CitationCapriotti AL, Caracciolo G, Caruso G, Cavaliere C, Pozzi D, et al. (2010) Analysis of plasma protein adsorption onto DC-Chol-DOPE cationic liposomes by HPLC-CHIP coupled to a Q-TOF mass spectrometer. Analytical and Bioanalytical Chemistry 398: 2895–2903. Available: http://dx.doi.org/10.1007/s00216-010-4104-y.
SponsorsThis publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).A special acknowledgment is given to Agilent Technologies and Agilent staff members, for their invaluable help and technical assistance. In particular, the authors wish to thank Alberto Stocco (Agilent Technologies) for his technical assistance and his infinite helpfulness.
CollectionsPublications Acknowledging KAUST Support
- Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface.
- Authors: Capriotti AL, Caracciolo G, Cavaliere C, Crescenzi C, Pozzi D, Laganà A
- Issue date: 2011 Sep
- Thermodynamic aspects and biological profile of CDAN/DOPE and DC-Chol/DOPE lipoplexes.
- Authors: Keller M, Jorgensen MR, Perouzel E, Miller AD
- Issue date: 2003 May 27
- DNA affects the composition of lipoplex protein corona: a proteomics approach.
- Authors: Capriotti AL, Caracciolo G, Caruso G, Foglia P, Pozzi D, Samperi R, Laganà A
- Issue date: 2011 Aug
- Surface adsorption of protein corona controls the cell internalization mechanism of DC-Chol-DOPE/DNA lipoplexes in serum.
- Authors: Caracciolo G, Callipo L, De Sanctis SC, Cavaliere C, Pozzi D, Laganà A
- Issue date: 2010 Mar
- DC-Chol/DOPE cationic liposomes: a comparative study of the influence factors on plasmid pDNA and siRNA gene delivery.
- Authors: Zhang Y, Li H, Sun J, Gao J, Liu W, Li B, Guo Y, Chen J
- Issue date: 2010 May 10