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dc.contributor.authorAli, Zahir
dc.contributor.authorAbulfaraj, Aala A.
dc.contributor.authorPiatek, Marek J.
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2016-02-25T12:33:28Z
dc.date.available2016-02-25T12:33:28Z
dc.date.issued2015-06-03
dc.identifier.citationAli Z, Abul-faraj A, Piatek M, Mahfouz MM (2015) Activity and specificity of TRV-mediated gene editing in plants. Plant Signaling & Behavior 10: e1044191. Available: http://dx.doi.org/10.1080/15592324.2015.1044191.
dc.identifier.issn1559-2324
dc.identifier.pmid26039254
dc.identifier.doi10.1080/15592324.2015.1044191
dc.identifier.urihttp://hdl.handle.net/10754/597449
dc.description.abstract© 2015 Taylor and Francis Group, LLC. Plant trait engineering requires efficient targeted genome-editing technologies. Clustered regularly interspaced palindromic repeats (CRISPRs)/ CRISPR associated (Cas) type II system is used for targeted genome-editing applications across eukaryotic species including plants. Delivery of genome engineering reagents and recovery of mutants remain challenging tasks for in planta applications. Recently, we reported the development of Tobacco rattle virus (TRV)-mediated genome editing in Nicotiana benthamiana. TRV infects the growing points and possesses small genome size; which facilitate cloning, multiplexing, and agroinfections. Here, we report on the persistent activity and specificity of the TRV-mediated CRISPR/Cas9 system for targeted modification of the Nicotiana benthamiana genome. Our data reveal the persistence of the TRVmediated Cas9 activity for up to 30 d post-agroinefection. Further, our data indicate that TRV-mediated genome editing exhibited no off-target activities at potential off-targets indicating the precision of the system for plant genome engineering. Taken together, our data establish the feasibility and exciting possibilities of using virus-mediated CRISPR/Cas9 for targeted engineering of plant genomes.
dc.description.sponsorshipWe would like to thank members of the laboratory for genome engineering at KAUST for helpful discussions and comments. This study is supported by King Abdullah University of Science and Technology (KAUST).
dc.publisherInforma UK Limited
dc.subjectCRISPR/Cas9 system
dc.subjectPlant genome engineering
dc.subjectSynthetic site-specific nucleases (SSNs)
dc.subjectTRV
dc.subjectViral-mediated genome editing
dc.titleActivity and specificity of TRV-mediated gene editing in plants
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Signaling & Behavior
dc.contributor.institutionKing Abdullah University of Science and Technology, Jeddah, Saudi Arabia
kaust.personAli, Zahir
kaust.personAbulfaraj, Aala Abdulaziz Hussien
kaust.personPiatek, Marek J.
kaust.personMahfouz, Magdy M.
kaust.acknowledged.supportUnitLaboratory for genome engineering
dc.date.published-online2015-06-03
dc.date.published-print2015-10-03


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