Show simple item record

dc.contributor.authorZubkov, V.S.
dc.contributor.authorCombes, A.N.
dc.contributor.authorShort, K.M.
dc.contributor.authorLefevre, J.
dc.contributor.authorHamilton, N.A.
dc.contributor.authorSmyth, I.M.
dc.contributor.authorLittle, M.H.
dc.contributor.authorByrne, H.M.
dc.date.accessioned2016-02-25T12:32:40Z
dc.date.available2016-02-25T12:32:40Z
dc.date.issued2015-08
dc.identifier.citationZubkov VS, Combes AN, Short KM, Lefevre J, Hamilton NA, et al. (2015) A spatially-averaged mathematical model of kidney branching morphogenesis. Journal of Theoretical Biology 379: 24–37. Available: http://dx.doi.org/10.1016/j.jtbi.2015.04.015.
dc.identifier.issn0022-5193
dc.identifier.pmid25913880
dc.identifier.doi10.1016/j.jtbi.2015.04.015
dc.identifier.urihttp://hdl.handle.net/10754/597410
dc.description.abstract© 2015 Published by Elsevier Ltd. Kidney development is initiated by the outgrowth of an epithelial ureteric bud into a population of mesenchymal cells. Reciprocal morphogenetic responses between these two populations generate a highly branched epithelial ureteric tree with the mesenchyme differentiating into nephrons, the functional units of the kidney. While we understand some of the mechanisms involved, current knowledge fails to explain the variability of organ sizes and nephron endowment in mice and humans. Here we present a spatially-averaged mathematical model of kidney morphogenesis in which the growth of the two key populations is described by a system of time-dependant ordinary differential equations. We assume that branching is symmetric and is invoked when the number of epithelial cells per tip reaches a threshold value. This process continues until the number of mesenchymal cells falls below a critical value that triggers cessation of branching. The mathematical model and its predictions are validated against experimentally quantified C57Bl6 mouse embryonic kidneys. Numerical simulations are performed to determine how the final number of branches changes as key system parameters are varied (such as the growth rate of tip cells, mesenchyme cells, or component cell population exit rate). Our results predict that the developing kidney responds differently to loss of cap and tip cells. They also indicate that the final number of kidney branches is less sensitive to changes in the growth rate of the ureteric tip cells than to changes in the growth rate of the mesenchymal cells. By inference, increasing the growth rate of mesenchymal cells should maximise branch number. Our model also provides a framework for predicting the branching outcome when ureteric tip or mesenchyme cells change behaviour in response to different genetic or environmental developmental stresses.
dc.description.sponsorshipThis work was supported by Human Frontiers Scientific Program (HFSP RGP0039/2011), the National Health and Medical Research Council, Australia (NHMMRC APP1002478; to ML), an Australian Research Council (ARC) Discovery Project Grant (DP130100886; to IS) and in part by Award No. KUK-C1-013-04 made by King Abdullah University of Science and Technology (KAUST). ML is a Senior Principal Research Fellow of the NHMRC. IS holds an ARC Future Fellowship. We are grateful to Professor Andrew McMahon and Dr Lori O׳Brien for helpful discussions and to Adler Ju for technical support.
dc.publisherElsevier BV
dc.subjectBranching morphogenesis
dc.subjectCap mesenchyme
dc.subjectKidney development
dc.subjectMathematical modeling
dc.subjectNephrogenesis
dc.subjectOrganogenesis
dc.subjectUreteric tree
dc.titleA spatially-averaged mathematical model of kidney branching morphogenesis
dc.typeArticle
dc.identifier.journalJournal of Theoretical Biology
dc.contributor.institutionUniversity of Oxford, Oxford, United Kingdom
dc.contributor.institutionUniversity of Queensland, Brisbane, Australia
dc.contributor.institutionMonash University, Melbourne, Australia
kaust.grant.numberKUK-C1-013-04


This item appears in the following Collection(s)

Show simple item record