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    A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement

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    Type
    Article
    Authors
    Lundahl, P. Johan
    Kitts, Catherine C.
    Nordén, Bengt
    Date
    2011
    Permanent link to this record
    http://hdl.handle.net/10754/597339
    
    Metadata
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    Abstract
    This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost. © 2011 The Royal Society of Chemistry.
    Citation
    Lundahl PJ, Kitts CC, Nordén B (2011) A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement. Analyst 136: 3303. Available: http://dx.doi.org/10.1039/c0an00455c.
    Sponsors
    This work was funded by King Abdullah University of Science and Technology (KAUST). Hanna Rydberg is acknowledged for supplying the liposome samples.
    Publisher
    Royal Society of Chemistry (RSC)
    Journal
    The Analyst
    ISSN
    0003-2654
    1364-5528
    DOI
    10.1039/c0an00455c
    PubMed ID
    21709867
    ae974a485f413a2113503eed53cd6c53
    10.1039/c0an00455c
    Scopus Count
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