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dc.contributor.authorCall, Douglas F.
dc.contributor.authorLogan, Bruce E.
dc.date.accessioned2016-02-25T12:30:15Z
dc.date.available2016-02-25T12:30:15Z
dc.date.issued2011-07
dc.identifier.citationCall DF, Logan BE (2011) A method for high throughput bioelectrochemical research based on small scale microbial electrolysis cells. Biosensors and Bioelectronics 26: 4526–4531. Available: http://dx.doi.org/10.1016/j.bios.2011.05.014.
dc.identifier.issn0956-5663
dc.identifier.pmid21652198
dc.identifier.doi10.1016/j.bios.2011.05.014
dc.identifier.urihttp://hdl.handle.net/10754/597304
dc.description.abstractThere is great interest in studying exoelectrogenic microorganisms, but existing methods can require expensive electrochemical equipment and specialized reactors. We developed a simple system for conducting high throughput bioelectrochemical research using multiple inexpensive microbial electrolysis cells (MECs) built with commercially available materials and operated using a single power source. MECs were small crimp top serum bottles (5mL) with a graphite plate anode (92m 2/m 3) and a cathode of stainless steel (SS) mesh (86m 2/m 3), graphite plate, SS wire, or platinum wire. The highest volumetric current density (240A/m 3, applied potential of 0.7V) was obtained using a SS mesh cathode and a wastewater inoculum (acetate electron donor). Parallel operated MECs (single power source) did not lead to differences in performance compared to non-parallel operated MECs, which can allow for high throughput reactor operation (>1000 reactors) using a single power supply. The utility of this method for cultivating exoelectrogenic microorganisms was demonstrated through comparison of buffer effects on pure (Geobacter sulfurreducens and Geobacter metallireducens) and mixed cultures. Mixed cultures produced current densities equal to or higher than pure cultures in the different media, and current densities for all cultures were higher using a 50mM phosphate buffer than a 30mM bicarbonate buffer. Only the mixed culture was capable of sustained current generation with a 200mM phosphate buffer. These results demonstrate the usefulness of this inexpensive method for conducting in-depth examinations of pure and mixed exoelectrogenic cultures. © 2011 Elsevier B.V.
dc.description.sponsorshipThis research was funded by the National Science Foundation Graduate Research Fellowship Program, the National Water Research Institute Ronald B. Linsky Fellowship, and award KUS-I1-003-13 from King Abdullah University of Science and Technology.
dc.publisherElsevier BV
dc.subjectBioelectrochemical system
dc.subjectGeobacter
dc.subjectMicrobial electrolysis cell
dc.titleA method for high throughput bioelectrochemical research based on small scale microbial electrolysis cells
dc.typeArticle
dc.identifier.journalBiosensors and Bioelectronics
dc.contributor.institutionPennsylvania State University, State College, United States
kaust.grant.numberKUS-I1-003-13


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