KAUST Grant NumberKUS-C1-016-04
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AbstractMotivation: Chromatin immunoprecipitation (ChIP) coupled with tiling microarray (chip) experiments have been used in a wide range of biological studies such as identification of transcription factor binding sites and investigation of DNA methylation and histone modification. Hidden Markov models are widely used to model the spatial dependency of ChIP-chip data. However, parameter estimation for these models is typically either heuristic or suboptimal, leading to inconsistencies in their applications. To overcome this limitation and to develop an efficient software, we propose a hidden ferromagnetic Ising model for ChIP-chip data analysis. Results: We have developed a simple, but powerful Bayesian hierarchical model for ChIP-chip data via a hidden Ising model. Metropolis within Gibbs sampling algorithm is used to simulate from the posterior distribution of the model parameters. The proposed model naturally incorporates the spatial dependency of the data, and can be used to analyze data with various genomic resolutions and sample sizes. We illustrate the method using three publicly available datasets and various simulated datasets, and compare it with three closely related methods, namely TileMap HMM, tileHMM and BAC. We find that our method performs as well as TileMap HMM and BAC for the high-resolution data from Affymetrix platform, but significantly outperforms the other three methods for the low-resolution data from Agilent platform. Compared with the BAC method which also involves MCMC simulations, our method is computationally much more efficient. Availability: A software called iChip is freely available at http://www.bioconductor.org/. Contact: firstname.lastname@example.org. © The Author 2010. Published by Oxford University Press. All rights reserved. For Permissions, please email: email@example.com.
CitationMo Q, Liang F (2010) A hidden Ising model for ChIP-chip data analysis. Bioinformatics 26: 777–783. Available: http://dx.doi.org/10.1093/bioinformatics/btq032.
SponsorsThe authors thank Hongkai Ji for helpful discussion about TileMap HMM, and the editor, the associate editor and the referees for their comments which have led to significant improvement of this article.Funding: National Science Foundation (grant DMS-0607755 to L.F., partially); award (KUS-C1-016-04) made by King Abdullah University of Science and Technology (KAUST to L.F.).
PublisherOxford University Press (OUP)
CollectionsPublications Acknowledging KAUST Support
- Bayesian modeling of ChIP-chip data through a high-order Ising model.
- Authors: Mo Q, Liang F
- Issue date: 2010 Dec
- rMAT--an R/Bioconductor package for analyzing ChIP-chip experiments.
- Authors: Droit A, Cheung C, Gottardo R
- Issue date: 2010 Mar 1
- A supervised hidden markov model framework for efficiently segmenting tiling array data in transcriptional and chIP-chip experiments: systematically incorporating validated biological knowledge.
- Authors: Du J, Rozowsky JS, Korbel JO, Zhang ZD, Royce TE, Schultz MH, Snyder M, Gerstein M
- Issue date: 2006 Dec 15
- TileMap: create chromosomal map of tiling array hybridizations.
- Authors: Ji H, Wong WH
- Issue date: 2005 Sep 15
- A hidden Markov model for analyzing ChIP-chip experiments on genome tiling arrays and its application to p53 binding sequences.
- Authors: Li W, Meyer CA, Liu XS
- Issue date: 2005 Jun