Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.
Heilig, Hans G H J
van Eekert, Miriam H A
Stams, Alfons J M
Rijpstra, Irene C
Sinninghe-Damsté, Jaap S
de Vos, Willem M
Kengen, Servé W M
KAUST Grant NumberKUK-C1-017-12
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AbstractA novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.
CitationBalk M, Heilig HGHJ, van Eekert MHA, Stams AJM, Rijpstra IC, et al. (2009) Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey. Extremophiles 13: 885–894. Available: http://dx.doi.org/10.1007/s00792-009-0276-9.
SponsorsThis work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST).
PubMed Central IDPMC2767516
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