Transcriptional repressor domain of MBD1 is intrinsically disordered and interacts with its binding partners in a selective manner.
AuthorsHameed, Umar Farook Shahul
Wasik, Mariusz A
Online Publication Date2014-05-09
Print Publication Date2015-05
Permanent link to this recordhttp://hdl.handle.net/10754/596829
MetadataShow full item record
AbstractMethylation of DNA CpG sites is a major mechanism of epigenetic gene silencing and plays important roles in cell division, development and carcinogenesis. One of its regulators is the 64-residue C-terminal Transcriptional Repressor Domain (the TRD) of MBD1, which recruits several repressor proteins such as MCAF1, HDAC3 and MPG that are essential for the gene silencing. Using NMR spectroscopy, we have characterized the solution structure of the C-terminus of MBD1 (MBD1-c, residues D507 to Q605), which included the TRD (A529 to P592). Surprisingly, the MBD1-c is intrinsically disordered. Despite its lack of a tertiary folding, MBD1-c could still bind to different partner proteins in a selective manner. MPG and MCAF1Δ8 showed binding to both the N-terminal and C-terminal residues of MBD1-c but HDAC3 preferably bound to the C-terminal region. This study reveals how MBD1-c discriminates different binding partners, and thus, expands our understanding of the mechanisms of gene regulation by MBD1.
CitationHameed UFS, Lim J, Zhang Q, Wasik MA, Yang D, et al. (2014) Transcriptional Repressor Domain of MBD1 is Intrinsically Disordered and Interacts with its Binding Partners in a Selective Manner. Scientific Reports 4. Available: http://dx.doi.org/10.1038/srep04896.
SponsorsWe appreciate the support of Profs. Rabindra Roy from Georgetown University, USA and Luciano DiCroce from Center for Genomic Regulation, Spain for providing the MPG and HDAC3 expression constructs, respectively. The authors thank the BioScience core Lab of King Abdullah University of Science and Technology (KAUST), Saudi Arabia for their support in performing ITC experiments. This project was supported by Academic Research Fund (FRC) of the Ministry of Education, Singapore to K.S. and National University of Singapore research scholarship to U.F.S.H.
PubMed Central IDPMC4014985
CollectionsPublications Acknowledging KAUST Support
Except where otherwise noted, this item's license is described as This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit
- The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.
- Authors: Reese BE, Bachman KE, Baylin SB, Rountree MR
- Issue date: 2003 May
- Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1.
- Authors: Fujita N, Shimotake N, Ohki I, Chiba T, Saya H, Shirakawa M, Nakao M
- Issue date: 2000 Jul
- Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters.
- Authors: Wischnewski F, Friese O, Pantel K, Schwarzenbach H
- Issue date: 2007 Jul
- Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.
- Authors: Fujita N, Takebayashi S, Okumura K, Kudo S, Chiba T, Saya H, Nakao M
- Issue date: 1999 Sep
- Active repression of methylated genes by the chromosomal protein MBD1.
- Authors: Ng HH, Jeppesen P, Bird A
- Issue date: 2000 Feb