The presence of hydrogenotrophic methanogens in the inoculum improves methane gas production in microbial electrolysis cells.
KAUST Grant NumberKUS-I1-003-13
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AbstractHigh current densities in microbial electrolysis cells (MECs) result from the predominance of various Geobacter species on the anode, but it is not known if archaeal communities similarly converge to one specific genus. MECs were examined here on the basis of maximum methane production and current density relative to the inoculum community structure. We used anaerobic digester (AD) sludge dominated by acetoclastic Methanosaeta, and an anaerobic bog sediment where hydrogenotrophic methanogens were detected. Inoculation using solids to medium ratio of 25% (w/v) resulted in the highest methane production rates (0.27 mL mL(-1) cm(-2), gas volume normalized by liquid volume and cathode projected area) and highest peak current densities (0.5 mA cm(-2)) for the bog sample. Methane production was independent of solid to medium ratio when AD sludge was used as the inoculum. 16S rRNA gene community analysis using pyrosequencing and quantitative PCR confirmed the convergence of Archaea to Methanobacterium and Methanobrevibacter, and of Bacteria to Geobacter, despite their absence in AD sludge. Combined with other studies, these findings suggest that Archaea of the hydrogenotrophic genera Methanobacterium and Methanobrevibacter are the most important microorganisms for methane production in MECs and that their presence in the inoculum improves the performance.
CitationSiegert M, Li X-F, Yates MD, Logan BE (2015) The presence of hydrogenotrophic methanogens in the inoculum improves methane gas production in microbial electrolysis cells. Frontiers in Microbiology 5. Available: http://dx.doi.org/10.3389/fmicb.2014.00778.
SponsorsThis research was supported by the Global Climate and Energy Program (GCEP) and the King Abdullah University of Science and Technology (KAUST, award KUS-I1-003-13).
PublisherFrontiers Media SA
JournalFrontiers in Microbiology
PubMed Central IDPMC4295556
CollectionsPublications Acknowledging KAUST Support
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