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dc.contributor.authorChailyan, Anna
dc.contributor.authorMarcatili, Paolo
dc.contributor.authorTramontano, Anna
dc.date.accessioned2016-02-21T08:51:20Z
dc.date.available2016-02-21T08:51:20Z
dc.date.issued2011-06-28
dc.identifier.citationChailyan A, Marcatili P, Tramontano A (2011) The association of heavy and light chain variable domains in antibodies: implications for antigen specificity. FEBS Journal 278: 2858–2866. Available: http://dx.doi.org/10.1111/j.1742-4658.2011.08207.x.
dc.identifier.issn1742-464X
dc.identifier.pmid21651726
dc.identifier.doi10.1111/j.1742-4658.2011.08207.x
dc.identifier.urihttp://hdl.handle.net/10754/596822
dc.description.abstractThe antigen-binding site of immunoglobulins is formed by six regions, three from the light and three from the heavy chain variable domains, which, on association of the two chains, form the conventional antigen-binding site of the antibody. The mode of interaction between the heavy and light chain variable domains affects the relative position of the antigen-binding loops and therefore has an effect on the overall conformation of the binding site. In this article, we analyze the structure of the interface between the heavy and light chain variable domains and show that there are essentially two different modes for their interaction that can be identified by the presence of key amino acids in specific positions of the antibody sequences. We also show that the different packing modes are related to the type of recognized antigen.
dc.description.sponsorshipThis work was partially supported by Award No. KUK-I1-012-43 made by the King Abdullah University of Science and Technology (KAUST), by Fondazione Roma and by the Italian Ministry of Health, contract no. onc_ord 25/07, FIRB ITALBIONET and PROTEOMICA.
dc.publisherWiley-Blackwell
dc.rightsRe-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/
dc.titleThe association of heavy and light chain variable domains in antibodies: implications for antigen specificity.
dc.typeArticle
dc.identifier.journalFEBS Journal
dc.identifier.pmcidPMC3562479
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italy
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italy
kaust.grant.numberKUK-I1-012-43
refterms.dateFOA2018-06-13T14:20:30Z


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Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
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