Immobilization of anode-attached microbes in a microbial fuel cell.
KAUST Grant NumberKUS-I1-003-13
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AbstractCurrent-generating (exoelectrogenic) bacteria in bioelectrochemical systems (BESs) may not be culturable using standard in vitro agar-plating techniques, making isolation of new microbes a challenge. More in vivo like conditions are needed where bacteria can be grown and directly isolated on an electrode. While colonies can be developed from single cells on an electrode, the cells must be immobilized after being placed on the surface. Here we present a proof-of-concept immobilization approach that allows exoelectrogenic activity of cells on an electrode based on applying a layer of latex to hold bacteria on surfaces. The effectiveness of this procedure to immobilize particles was first demonstrated using fluorescent microspheres as bacterial analogs. The latex coating was then shown to not substantially affect the exoelectrogenic activity of well-developed anode biofilms in two different systems. A single layer of airbrushed coating did not reduce the voltage produced by a biofilm in a microbial fuel cell (MFC), and more easily applied dip-and-blot coating reduced voltage by only 11% in a microbial electrolysis cell (MEC). This latex immobilization procedure will enable future testing of single cells for exoelectrogenic activity on electrodes in BESs.
CitationWagner RC, Porter-Gill S, Logan BE (2012) Immobilization of anode-attached microbes in a microbial fuel cell. AMB Express 2: 2. Available: http://dx.doi.org/10.1186/2191-0855-2-2.
SponsorsThis material is based upon work supported under National Science Foundation Graduate Research Fellowships (RCW), and award KUS-I1-003-13 by King Abdullah University of Science and Technology (KAUST).
PubMed Central IDPMC3311593
CollectionsPublications Acknowledging KAUST Support
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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