Convergent development of anodic bacterial communities in microbial fuel cells.
Type
ArticleAuthors
Yates, Matthew DKiely, Patrick D
Call, Douglas F
Rismani-Yazdi, Hamid
Bibby, Kyle
Peccia, Jordan
Regan, John M
Logan, Bruce E
KAUST Grant Number
KUS-I1-003-13Date
2012-05-10Permanent link to this record
http://hdl.handle.net/10754/596779
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Show full item recordAbstract
Microbial fuel cells (MFCs) are often inoculated from a single wastewater source. The extent that the inoculum affects community development or power production is unknown. The stable anodic microbial communities in MFCs were examined using three inocula: a wastewater treatment plant sample known to produce consistent power densities, a second wastewater treatment plant sample, and an anaerobic bog sediment. The bog-inoculated MFCs initially produced higher power densities than the wastewater-inoculated MFCs, but after 20 cycles all MFCs on average converged to similar voltages (470±20 mV) and maximum power densities (590±170 mW m(-2)). The power output from replicate bog-inoculated MFCs was not significantly different, but one wastewater-inoculated MFC (UAJA3 (UAJA, University Area Joint Authority Wastewater Treatment Plant)) produced substantially less power. Denaturing gradient gel electrophoresis profiling showed a stable exoelectrogenic biofilm community in all samples after 11 cycles. After 16 cycles the predominance of Geobacter spp. in anode communities was identified using 16S rRNA gene clone libraries (58±10%), fluorescent in-situ hybridization (FISH) (63±6%) and pyrosequencing (81±4%). While the clone library analysis for the underperforming UAJA3 had a significantly lower percentage of Geobacter spp. sequences (36%), suggesting that a predominance of this microbe was needed for convergent power densities, the lower percentage of this species was not verified by FISH or pyrosequencing analyses. These results show that the predominance of Geobacter spp. in acetate-fed systems was consistent with good MFC performance and independent of the inoculum source.Citation
Yates MD, Kiely PD, Call DF, Rismani-Yazdi H, Bibby K, et al. (2012) Convergent development of anodic bacterial communities in microbial fuel cells. ISME J 6: 2002–2013. Available: http://dx.doi.org/10.1038/ismej.2012.42.Sponsors
This research was supported by Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and by Award DGE-0750756 from the National Science Foundation (NSF) Graduate Student Fellowship Program.Publisher
Springer NatureJournal
The ISME JournalISSN
1751-73621751-7370
PubMed ID
22572637PubMed Central ID
PMC3475369ae974a485f413a2113503eed53cd6c53
10.1038/ismej.2012.42
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