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dc.contributor.authorSo, Hongyun
dc.contributor.authorLee, Kunwoo
dc.contributor.authorMurthy, Niren
dc.contributor.authorPisano, Albert P
dc.date.accessioned2016-02-21T08:50:13Z
dc.date.available2016-02-21T08:50:13Z
dc.date.issued2014-11-24
dc.identifier.citationSo H, Lee K, Murthy N, Pisano AP (2014) All-in-One Nanowire-Decorated Multifunctional Membrane for Rapid Cell Lysis and Direct DNA Isolation. ACS Applied Materials & Interfaces 6: 20693–20699. Available: http://dx.doi.org/10.1021/am506153y.
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.pmid25420232
dc.identifier.doi10.1021/am506153y
dc.identifier.urihttp://hdl.handle.net/10754/596765
dc.description.abstractThis paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.
dc.description.sponsorshipThis work was partially supported by the grants from the KAUST Global Collaborative Research under the Academic Excellence Alliance program, the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in the Nanotechnology award (HHSN268201000043C), RO1HL096796-02, and RO1AI088023-03. The authors are also grateful for support from Marvell Nanolab and Biomolecular Nanotechnology Center at UC Berkeley where all devices were fabricated. H.S. greatly acknowledges Prof. Young Ho Seo at Kangwon National University in Korea for his valuable discussion.
dc.publisherAmerican Chemical Society (ACS)
dc.rightsThis is an open access article published under an ACS AuthorChoice , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.subjectDNA purification
dc.subjectcell lysis
dc.subjectNanowires
dc.subjectPoint-of-care Diagnostics
dc.subjectPorous Silicon Membrane
dc.titleAll-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.
dc.typeArticle
dc.identifier.journalACS Applied Materials & Interfaces
dc.identifier.pmcidPMC4264858
dc.contributor.institutionDepartment of Mechanical Engineering, Berkeley Sensor & Actuator Center and ‡Department of Bioengineering, University of California , Berkeley, California 94720, United States.
kaust.grant.programAcademic Excellence Alliance (AEA)
refterms.dateFOA2018-06-13T12:03:53Z
dc.date.published-online2014-11-24
dc.date.published-print2014-12-10


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