All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.
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ArticleDate
2014-11-24Online Publication Date
2014-11-24Print Publication Date
2014-12-10Permanent link to this record
http://hdl.handle.net/10754/596765
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This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.Citation
So H, Lee K, Murthy N, Pisano AP (2014) All-in-One Nanowire-Decorated Multifunctional Membrane for Rapid Cell Lysis and Direct DNA Isolation. ACS Applied Materials & Interfaces 6: 20693–20699. Available: http://dx.doi.org/10.1021/am506153y.Sponsors
This work was partially supported by the grants from the KAUST Global Collaborative Research under the Academic Excellence Alliance program, the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in the Nanotechnology award (HHSN268201000043C), RO1HL096796-02, and RO1AI088023-03. The authors are also grateful for support from Marvell Nanolab and Biomolecular Nanotechnology Center at UC Berkeley where all devices were fabricated. H.S. greatly acknowledges Prof. Young Ho Seo at Kangwon National University in Korea for his valuable discussion.Publisher
American Chemical Society (ACS)PubMed ID
25420232PubMed Central ID
PMC4264858ae974a485f413a2113503eed53cd6c53
10.1021/am506153y
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