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dc.contributor.authorLi, Bo
dc.contributor.authorByrt, Caitlin
dc.contributor.authorQiu, Jiaen
dc.contributor.authorBaumann, Ute
dc.contributor.authorHrmova, Maria
dc.contributor.authorEvrard, Aurelie
dc.contributor.authorJohnson, Alexander A T
dc.contributor.authorBirnbaum, Kenneth D.
dc.contributor.authorMayo, Gwenda M.
dc.contributor.authorJha, Deepa
dc.contributor.authorHenderson, Sam W
dc.contributor.authorTester, Mark A.
dc.contributor.authorGilliham, Mathew
dc.contributor.authorRoy, Stuart J.
dc.date.accessioned2016-02-10T13:21:23Z
dc.date.available2016-02-10T13:21:23Z
dc.date.issued2015-12-11
dc.identifier.citationLi, B., Byrt, C.S., Qiu, J., Baumann, U., Hrmova, M., Evrard, A., Johnson, A.A., Birnbaum, K.D., Mayo, G.M., Jha, D. and Henderson, S.W., 2015. Identification of a stelar-localised transport protein that facilitates root-to-shoot transfer of chloride in Arabidopsis. Plant physiology, pp.pp-01163.
dc.identifier.issn0032-0889
dc.identifier.pmid26662602
dc.identifier.doi10.1104/pp.15.01163
dc.identifier.urihttp://hdl.handle.net/10754/596021
dc.description.abstractUnder saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in the shoot by regulating their transfer from the root symplast into the xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen of salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate gene involved in Cl– xylem loading from the Nitrate transporter 1/Peptide Transporter family (NPF2.4). This gene was highly expressed in the root stele compared to the cortex, and its expression decreased after exposure to NaCl or abscisic acid. NPF2.4 fused to fluorescent proteins, expressed either transiently or stably, was targeted to the plasma membrane. Electrophysiological analysis of NPF2.4 in Xenopus laevis oocytes suggested that NPF2.4 catalyzed passive Cl– efflux out of cells and was much less permeable to NO3−. Shoot Cl– accumulation was decreased following NPF2.4 artificial microRNA knockdown, whereas it was increased by overexpression of NPF2.4. Taken together, these results suggest that NPF2.4 is involved in long-distance transport of Cl– in plants, playing a role in the loading and the regulation of Cl– loading into the xylem of Arabidopsis roots during salinity stress.
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.relation.urlhttp://www.plantphysiol.org/content/170/2/1014
dc.rightsArchived with thanks to Plant Physiology
dc.titleIdentification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis
dc.typeArticle
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentElectrical Engineering Program
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalPlant Physiology
dc.eprint.versionPost-print
dc.contributor.institutionAustralian Centre for Plant Functional Genomics
dc.contributor.institutionARC Centre of Excellence in Plant Energy Biology
dc.contributor.institutionUniversity of Adelaide, SA 5064, Australia
dc.contributor.institutionSchool of BioSciences, University of Melbourne, Parkville, Vic 3010, Australia
dc.contributor.institutionCentre for Genomics and Systems Biology, New York University, New York 10003
dc.contributor.institutionSchool of Agriculture, Food, and Wine
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personShamma, Jeff S.
refterms.dateFOA2016-12-11T00:00:00Z
dc.date.published-online2015-12-11
dc.date.published-print2016-02


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