Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis
Johnson, Alexander A T
Birnbaum, Kenneth D.
Mayo, Gwenda M.
Henderson, Sam W
Tester, Mark A.
Roy, Stuart J.
KAUST DepartmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Desert Agriculture Initiative
Online Publication Date2015-12-11
Print Publication Date2016-02
Permanent link to this recordhttp://hdl.handle.net/10754/596021
MetadataShow full item record
AbstractUnder saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in the shoot by regulating their transfer from the root symplast into the xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen of salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate gene involved in Cl– xylem loading from the Nitrate transporter 1/Peptide Transporter family (NPF2.4). This gene was highly expressed in the root stele compared to the cortex, and its expression decreased after exposure to NaCl or abscisic acid. NPF2.4 fused to fluorescent proteins, expressed either transiently or stably, was targeted to the plasma membrane. Electrophysiological analysis of NPF2.4 in Xenopus laevis oocytes suggested that NPF2.4 catalyzed passive Cl– efflux out of cells and was much less permeable to NO3−. Shoot Cl– accumulation was decreased following NPF2.4 artificial microRNA knockdown, whereas it was increased by overexpression of NPF2.4. Taken together, these results suggest that NPF2.4 is involved in long-distance transport of Cl– in plants, playing a role in the loading and the regulation of Cl– loading into the xylem of Arabidopsis roots during salinity stress.
CitationLi, B., Byrt, C.S., Qiu, J., Baumann, U., Hrmova, M., Evrard, A., Johnson, A.A., Birnbaum, K.D., Mayo, G.M., Jha, D. and Henderson, S.W., 2015. Identification of a stelar-localised transport protein that facilitates root-to-shoot transfer of chloride in Arabidopsis. Plant physiology, pp.pp-01163.
- SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl- accumulation and salt tolerance in Arabidopsis thaliana.
- Authors: Qiu J, Henderson SW, Tester M, Roy SJ, Gilliham M
- Issue date: 2016 Aug
- Allantoin accumulation mediated by allantoinase downregulation and transport by Ureide Permease 5 confers salt stress tolerance to Arabidopsis plants.
- Authors: Lescano CI, Martini C, González CA, Desimone M
- Issue date: 2016 Jul
- Chloride on the Move.
- Authors: Li B, Tester M, Gilliham M
- Issue date: 2017 Mar
- Vacuolar Chloride Fluxes Impact Ion Content and Distribution during Early Salinity Stress.
- Authors: Baetz U, Eisenach C, Tohge T, Martinoia E, De Angeli A
- Issue date: 2016 Oct
- Nitrate-Dependent Control of Shoot K Homeostasis by the Nitrate Transporter1/Peptide Transporter Family Member NPF7.3/NRT1.5 and the Stelar K+ Outward Rectifier SKOR in Arabidopsis.
- Authors: Drechsler N, Zheng Y, Bohner A, Nobmann B, von Wirén N, Kunze R, Rausch C
- Issue date: 2015 Dec